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16
PERSONALIZE YOUR PIPETMAN® M
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PIPETMAN® M
General Guidelines for Good Pipetting
●
Make sure that you fit new tips.
●
Each new tip should be pre-wetted with the liquid to be pipetted.
●
When aspirating, keep the tip at a constant depth below the surface of the liquid
(refer to table 1 on page 15).
●
Change the tip before aspirating a different liquid, sample, or reagent.
●
Change the tip if a droplet remains at the end of the tip from the previous pipetting
operation.
●
Liquid should never enter the tip holder. To prevent this:
○
Never turn the pipette upside down,
○
Never lay the pipette on its side when there is liquid in the tip(s),
The Gilson Stand Adapter (refer to Chapter 2
Parts Checklist and Accessories
on page 5)
is recommended for use with the CARROUSEL
TM
and SINGLE
TM
Pipette Holder to store
your PIPETMAN M pipette in the vertical position. Alternatively the POWER CARROUSEL
TM
can be used to store and charge up to five PIPETMAN M pipettes.
●
When pipetting liquids with temperatures different to the ambient temperature,
pre-we the tip several times before use in order to reach equilibrium between the
temperatures of the liquid and the pipette’s dead volume.
●
For volatile liquids, saturate the dead volume by aspirating and dispensing the liquid
repeatedly before aspirating the sample.
After pipetting acids or other corrosive liquids that emit vapors, clean the pipette, as
on page 26.
The pipette can be used between 4°C and 40°C, but the specifications may vary (refer to
Chapter 18
on page 29).
●
Do not pipette liquids at temperatures above 50°C or below 4°C.
Chapter 10
PERSONALIZE YOUR PIPETMAN® M
Tip Ejector Extension for Use with P10M
Tip ejector extensions is required to eject D10 tips and is supplied with P10M pipettes.
The tip ejector extension which is made of PVDF
(polyvinylidene Fluoride), is autoclavable.
To fit a tip ejector extension:
1.
Hold the pipette with the push button upright.
2. Hold the extension with the slot upright.
3. Slide the extension over the tip holder.
4. Push the extension firmly onto the end of the tip
ejector until it clicks into place (see opposite).
To fit
To remove
Slot
NOTE
Extreme temperatures can affect accuracy and precision.
Figure 9
Fit a tip ejector
