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CONFIGURATION
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PIPETMAN® M
Adjustment
Press the mode button to enter the menu. Select
“Configuration” by rotating the push
button and click to confirm your selection. Enter the sub-menu in the same way and
select the
“Adjustment” menu.
The adjustment menu allows you to access the following items:
●
Standard readjustment
●
Reset settings
Standard Readjustment
This menu allows you to adjust the pipette using three calibration points: 10%, 50%,
and 100% of nominal volume (in according with ISO8655 recommendations).
You may want to calibrate your pipette for solutions with a density, viscosity, surface
tension or vapour pressure that are different than that of water. To return to the
factory settings, choose
Reset settings
. Your PIPETMAN M will be reset to the three
factory calibration values that are permanently stored in the pipette’s firmware.
In accordance with ISO 8655 Gilson recommends a gravimetric procedure for
pipette calibration. This gravimetric method is used to establish the mean mass
of a given volume of water (taking into account evaporation losses, where
necessary). After converting the mean mass to a volume (using the Z factor, refer to
on page 52), enter the measured volumes(s) into the pipette’s
memory and the software readjusts the pipette accordingly. This method requires
the strict monitoring of environmental conditions and the use of routinely controlled
equipment that is adapted to the volume being measured.
Conversion to volume must take into account the density of the liquid as well as
evaporation during the cycle time. For each measurement, the corresponding volume
(
Vi
) can be calculated as follows:
Wi
is the weight as read on the balance
e
is the mean evaporation loss during the cycle
time
Z
expressed in L/mg, is a conversion factor incorporating density of water buoyed in
air, at test temperature and barometric pressure (refer to
on
page 52)
For volumes greater than 20 μL, the evaporation factor can be disregarded.
For more information please refer to
“Verification procedure for accuracy and
precision” which you can download from the Gilson website (
).
Maintenance Operation
Disassembly of your PIPETMAN M to Change and Clean Parts
It is best to inspect your pipette regularly and to routinely clean and change parts
as required. To help you to keep up a regular schedule and in the interests of good
laboratory practices (GLP), you can configure your pipette to display an alarm before
servicing is due (refer to page 18).
Vi = (Wi + e) Z
NOTE
The following maintenance operation should only be done when the
pipette is in DISASSEMBLY Mode.
