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30

LEAK TEST

 

PIPETMAN® M

The adjustment is carried out under strictly defined and monitored conditions (ISO 8655-6):

 

Basis of adjustment, Ex.

 

Reference temperature, 20°C

 

Relative humidity, 50%

 

Barometric pressure, 101 kPa

 

Use of distilled water grade 3 (ISO 3696)

 

Ten measurements for each test 

volume, which are nominal volume, 

50% of nominal volume and 10% 

of nominal volume. (tested mode: 

PIPET, speed 6 with  

PIPETMAN DIAMOND Tips)

 

PIPETMAN M SINGLE CHANNEL — VARIABLE VOLUME MODELS

Model

PIPETMAN 

DIAMOND Tips

Part 

Number

Gilson Specifications

I

SO 8655-2

Standard PIPET mode

REPETITIVE 

mode 

volume

Systematic  
error (µL)

Random 
 error (µL)

Volume Range

Volume 

(μL)

Systematic 
error (μL)

Random 
error (μL)

P10M

D10

DL10

DF10

DFL10

F81022

0.5–10 µL

0.5

1

5

10

± 0.040

± 0.025

± 0.060

± 0.080

 0.013

 0.012

 0.020

 0.025

0.5–10 µL

± 0.12

± 0.12

± 0.12

± 0.12

 0.08

 0.08

 0.08

 0.08

P20M

D200

DF30

F81023

2–20 µL

2

10

20

± 0.075

± 0.100

± 0.150

 0.025

 0.035

 0.050

2–20 µL

± 0.2

± 0.2

± 0.2

± 0.2

 0.1

 0.1

 0.1

 0.1

P100M

D200

D100

F81013

5–100 µL

5

10

50

100

± 0.35

± 0.30

± 0.38

± 0.40

 0.10

 0.10

 0.12

 0.15

5–100 µL

± 0.8

± 0.8

± 0.8

± 0.8

 0.3

 0.3

 0.3

 0.3

P200M

D200

D300

DF200

DF300

F81024

20–200 µL

20

100

200

± 0.40

± 0.80

± 1.00

 0.15

 0.22

 0.26

5 – 200 µL

± 1.6

± 1.6

± 1.6

 0.6

 0.6

 0.6

P300M

D200

D300

DF200

DF300

F81014

20–300 µL

20

30

150

300

± 0.80

± 0.70

± 0.90

± 1.05

 0.16

 0.20

 0.23

 0.30

10–300 µL

± 4.0

± 4.0

± 4.0

± 4.0

 1.5

 1.5

 1.5

 1.5

P1200M

D1000

D1200

DF1000

DF1200

F81015

100–1200 µL

100

120

600

1200

± 2.5

± 2.4

± 3.6

± 6.0

 0.4

 0.4

 0.8

 1.2

20–1200 µL

± 16

± 16

± 16

± 16

 6.0

 6.0

 6.0

 6.0

P5000M

D5000

F81016

500–5000 µL

500

2500

5000

± 10

± 15

± 25

 2

 4

 7

100–5000 µL

± 40

± 40

± 40

 15.0

 15.0

 15.0

P10mLM

D10mL

F81017

1–10 mL

1 mL 

5 mL

10 mL

± 25

± 30

± 50

 4

 8

 12

200 µL–10 mL

± 60

± 60

± 60

 30.0

 30.0

 30.0

 

 

PIPETMAN M MULTICHANNEL

P8x10M

D10 

DL10

DF10 

DFL10

F81025

0.5–10 µL

0.5

1

5

10

± 0.05

± 0.04

± 0.08

± 0.10

 0.02

 0.02

 0.04

 0.06

0.5–10 µL

± 0.24

± 0.24

± 0.24

± 0.24

 0.16

 0.16

 0.16

 0.16

P12x10M

F81026

P8x20M

DL10

D200

DFL10

DF30

F81027

1–20 µL

1

2

10

20

± 0.08

± 0.09

± 0.15

± 0.25

 0.05

 0.06

 0.10

 0.12

1–20 µL

± 0.4

± 0.4

± 0.4

± 0.4

 0.2

 0.2

 0.2

 0.2

P12x20M

F81028

P8x100M

D200 

DF100

F81018

10–100 µL

10

50

100

± 0.25

± 0.50

± 0.80

 0.14

 0.20

 0.25

5–100 µL

± 1.6

± 1.6

± 1.6

 0.6

 0.6

 0.6

P12x100M

F81019

P8x200M

D200 

D300

DF100

DF200 

DF300

F81029

20–200 µL

20

100

200

± 0.50

± 1.00

± 2.00

 0.16

 0.30

 0.50

5–200 µL

± 3.2

± 3.2

± 3.2

 1.2

 1.2

 1.2

P12x200M

F81030

P8x300M

D200 

D300

DF200 

DF300

F81031

10–300 µL

10

30

150

300

± 1.00

± 1.00

± 1.50

± 2.40

 0.18

 0.18 

 0.375

 0.45

10–300 µL

± 8.0

± 8.0

± 8.0

± 8.0

 3.0

 3.0

 3.0

 3.0

P12x300M

F81032

P8x1200M

D1200

DF1200

F81020

50–1200 µL

50

120

600

1200

± 4.0

± 4.0

± 6.0

± 9.6

 0.7

 0.7

 1.5

 1.8

50–1200 µL

± 32

± 32

± 32

± 32

 12

 12

 12

 12

P12x1200M

F81021

Table 2

PIPETMAN® M maximum permissible errors 

 Gilson maximum permissible errors are guaranteed only when PIPETMAN pipettes are used with the recommended  
PIPETMAN DIAMOND Tips. 

Under these conditions: 

 

Gilson volumetric specifications in standard pipetting (PIPET mode) are guaranteed with a performance exceeding  

ISO 8655-2 recommendations for this mode.

 

In the absence of ISO recommendations for repetitive pipetting mode for air-displacement pipettes, Gilson volumetric 

specifications for repetitive pipetting (REPETITIVE mode) are guaranteed within ISO 8655-2 recommendations for standard 

pipetting (Cf. ISO 8655-2 table 2 above).

Summary of Contents for F81013

Page 1: ...User s Guide EN...

Page 2: ...8 MIX Mode 9 REVERSE Mode 11 CUSTOM Mode 12 PIPETTING SPEED CONTROL 13 PIPETMAN DIAMOND TIPS 13 GUIDELINES FOR GOOD PIPETTING 15 Aspirate and Dispense 15 Pre Wet the Tips 15 General Guidelines for Goo...

Page 3: ...TEST 28 TROUBLESHOOTING 29 SPECIFICATIONS 29 SAFETY PRECAUTIONS AND LIMITATIONS OF USE 31 REPLACEMENT PARTS 32 Single Channel Models 32 Multichannel Models 32 PIPETMAN M SOFTWARE 33 What is PIPETMAN M...

Page 4: ...of applications standard pipetting repetitive mix reverse and custom mode Minimal effort aspirate and dispense with one click of the push button and eject tips with ease to help reduce the risk of rep...

Page 5: ...attery capacity in less than an hour it takes three hours to fully charge the battery Please refer to Chapter 12 Power Management on page 18 for charging procedure Switch on your PIPETMAN M Press the...

Page 6: ...forces removable for cleaning and servicing Ejector clip Ejector support Cover Ejector spacer Screen Pipetting Mode Aspirate and dispense indicator Battery status Purge indicator Volume 1 2 3 4 5 6 7...

Page 7: ...Adjust the Volume Setting 1 Hold your PIPETMAN M in a nearly vertical position 2 Turn the push button half way The screen will display Click to change volume 3 Press the push button the volume on the...

Page 8: ...tically calculates the number of aliquots possible from the nominal maximum volume NV of the pipette as follows N NV AV e g 120 L x 10 for P1200M You also can decrease the number of repetitions e g 12...

Page 9: ...mode forward pipetting followed by a mixing phase composed of repeatedly aspirating and dispensing as well as an optional forward pipetting step 1 Press mode button to access menu where you can choose...

Page 10: ...reset takes place after you release the push button again This feature enables you to remove the tip from the liquid without aspirating anything Aspirate and Dispense the Second Volume VOLUME 2 repea...

Page 11: ...REVERSE Mode by turning the push button To confirm your selection click the push button Set the volume 1 Press the push button set the volume by turning the push button and press again to confirm you...

Page 12: ...or export custom protocols easily and rapidly To create a protocol choose tasks in PIPETMAN M programmable software Aspirate aspirates specified volume into the tip Beep makes pipette produce a beep s...

Page 13: ...highest specifications strict quality control is maintained throughout the manufacturing process These tips are used to calibrate PIPETMAN M therefore for optimum performance we recommend using PIPETM...

Page 14: ...ions PIPETMAN M MULTICHANNEL P8x10M D10 DL10 DF10 DFL10 0 5 10 L P12x10M P8x20M DL10 D200 DFL10 DF30 1 20 L P12x20M P8x100M D200 DF100 10 100 L P12x100M P8x200M D200 D300 DF100 DF200 DF300 20 200 L P1...

Page 15: ...te vertically and immerse the tip in the liquid refer to page 14 Press the push button to aspirate the set volume of liquid Wait a few seconds time depends on model refer to page 14 then withdraw the...

Page 16: ...re pre we the tip several times before use in order to reach equilibrium between the temperatures of the liquid and the pipette s dead volume For volatile liquids saturate the dead volume by aspiratin...

Page 17: ...Y PRACTICE FEATURES Your PIPETMAN M is fully compliant to ISO8655 standard and is CE marked for EMC and LDV directives PIPETMAN M includes the following good laboratory practice GLP features General L...

Page 18: ...e power supply 1 Connect the AC adapter to a suitable AC power supply 2 Plug the adapter into the USB port of the pipette The pipette starts charging Charge your pipette by using stand adapter refer t...

Page 19: ...en a batt too low message will appear The pipette will display the last settings used a few minutes later and will continue charging Changing the Battery If the following symptoms occur please contact...

Page 20: ...our pipette Beeper You can switch the tone of your pipette on or off Contrast You can choose a value of 1 to 5 to set up the contrast of the screen Service refer to Chapter 14 Maintenance below Adjust...

Page 21: ...en appears Now you can remove the lower part of your pipette with complete security Service info provides you with maintenance information you cannot modify any of the items Number of pipetting cycles...

Page 22: ...umes s into the pipette s memory and the software readjusts the pipette accordingly This method requires the strict monitoring of environmental conditions and the use of routinely controlled equipment...

Page 23: ...away from the body of the pipette 4 Clean or autoclave the tip ejector and refit or replace it by reversing the procedure Changing the Tip Holder Lower Part After removing the tip ejector you may remo...

Page 24: ...ove the lower part take care to remove the O ring as described in Changing the Seal and or O ring above 2 Unscrew the connecting nut turn by hand counterclockwise 3 Gently remove the connecting nut an...

Page 25: ...nly lubricated and that you wipe away any excess remember only a fine film of lubricant is required over the entire piston Maintenance Operation for Multichannel Models For multichannel models the low...

Page 26: ...ETMAN M you may use a simple soap solution or any of the solutions mentioned in Decontamination Procedures for Gilson Pipettes Single Channel Models External 1 Remove the tip ejector for cleaning refe...

Page 27: ...mination Autoclaving Single Channel Models However after separation from the body any of the following components of the volumetric module may be autoclaved individually tip ejector connecting nut tip...

Page 28: ...r sterile water 4 Leave the parts to dry by evaporation or wipe them with a clean lint free tissue or soft cloth 5 Lubricate the piston and reassemble the pipette according to the instructions given i...

Page 29: ...BLEM POSSIBLE CAUSE PAGE Pipette is not precise Unscrewed lower part of tip holder 24 Incorrect operator technique 15 Worn O ring 24 Connecting nut is loose 24 25 Damaged or corroded piston 24 Damaged...

Page 30: ...500 5000 L 500 2500 5000 10 15 25 2 4 7 100 5000 L 40 40 40 15 0 15 0 15 0 P10mLM D10mL F81017 1 10 mL 1 mL 5 mL 10 mL 25 30 50 4 8 12 200 L 10 mL 60 60 60 30 0 30 0 30 0 PIPETMAN M MULTICHANNEL P8x1...

Page 31: ...information about where you can drop off your waste equipment for recycling please contact your local dealer from whom you originally purchased the product or your local council By doing so you will...

Page 32: ...F807020 F807128 F807129 Chapter 20 REPLACEMENT PARTS DESCRIPTION P8X10M P12X10M P8X20M P12X20M P8X100M P12X100M P8X200M P12X200M P8X300M P12X300M P8X1200M 12X1200M Battery tag 1x4 F807013 F807013 F80...

Page 33: ...can also select I don t want to use passwords You can still switch to start using passwords later in main menu select Other administration uChange password User Profile Provide Institution Company det...

Page 34: ...ipette settings Access additional pipette settings such as locking functionality sound alerts maximum volume limit or pipette firmware updates In the case of a forgotten password click the Forgotten P...

Page 35: ...ick the PIPETMAN M icon on the desktop 5 Once installed the PIPETMAN M Software can be run by any user on the installed PC Other administration Manage the inventory of your PIPETMAN M pipettes view hi...

Page 36: ...the screen to the Protocol pane on the right of the screen and modifying the task s default parameters 1 Select a task in the Available task list 2 Drag and drop it to the Protocol pane use the arrow...

Page 37: ...h drag and drop actions by selecting a task and clicking the arrow button or by double clicking the task Drag and drop actions position the tasks in the following way the selected task will be placed...

Page 38: ...specify the name of the task Speed 2 specify the speed for the dispense Comments enter any comments related to the task Display text 2 enter text that is displayed on the pipette s LCD screen when th...

Page 39: ...d NOTE The number of characters for Display text is limited 3 Purge task has the following parameters Task name 2 specify the name of the task Comments enter any comments related to the task Display t...

Page 40: ...and store them You can clone a task by right clicking on the desired task and selecting Clone task Add task window opens where you can rename the task and change the parameters if desired When done a...

Page 41: ...details about the warning are available if you position the mouse over the warning sign Such protocols may be transferred to a pipette and can be exported However executing these protocols on the pip...

Page 42: ...o fit volume specifications of the new PIPETMAN M model Transferring Custom Protocols to the Pipette To transfer a protocol to a PIPETMAN M pipette click the Transfer custom protocols to pipette butto...

Page 43: ...appears which informs you that the pipette is not yet registered Here you can click on Add pipette now which takes you to the pipette management screen or you can proceed to stay at step 1 by clickin...

Page 44: ...onfiguration Speed and Mode on PIPETMAN M pipette Set Maximal volume limit this sets the maximal allowed volume on the particular pipette it cannot be higher than pipette s specified maximal volume Si...

Page 45: ...ior to transferring custom protocols If the connected pipette is not yet registered a pop up appears which informs you that the pipette is not yet registered Here you can click on Add pipette now whic...

Page 46: ...y again button If a pipette is already correctly connected when Register new pipette button is clicked this step is skipped 2 Type in the name of the pipette and click Finish 3 Pipette is now listed i...

Page 47: ...interval will be listed in the table below displaying the following information for each protocol Protocole name Pipette name Pipette model Pipette serial Uploaded on and Result To display history of...

Page 48: ...nstitution Company Profile Enter the information related to your Institution Company here or modify it Simply enter the information in provided fields and click Save None of the information is mandato...

Page 49: ...P12x20M P8x100M P12x100M P8x200M P12x200M P8x300M P12x300M P8x1200M P12x1200M Comply with the requirements of the following standards Standards for EU Electromagnetic Compatibility Directive 2014 30 E...

Page 50: ...ent This warranty shall also be void in the event pipettes are altered or modified by any party other than Gilson or its designates Gilson s sole liability under this warranty shall be limited to at G...

Page 51: ...r the calculation w10 0 967 mg W _ W 0 968 0 960 0 984 0 942 0 969 0 966 0 955 0 972 0 958 0 967 10 _ W 0 964 mg _ n number of weighings Wi weighing results Wi 1 n n i 1 4 Calculate the mean volume Fo...

Page 52: ...1 0025 1 0025 1 0026 1 0026 19 1 0024 1 0025 1 0025 1 0026 1 0027 1 0027 19 5 1 0025 1 0026 1 0026 1 0027 1 0028 1 0028 20 1 0026 1 0027 1 0027 1 0028 1 0029 1 0029 20 5 1 0027 1 0028 1 0028 1 0029 1...

Page 53: ...using a pair of tweezers 2 Aspirate a sample 3 Tare the balance and take the weighing vessel out of the balance 4 Take off the lid with tweezers 5 Dispense the sample into a dummy vessel 6 Replace th...

Page 54: ...gilson com contactus LT801563 D 2021 Gilson Inc All rights reserved...

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