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DESCRIPTION
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PIPETMAN® M
3. Set the
MIX
volume: press the push button, set the volume by turning the push
button. Confirm your settings with a simple click. This is the volume which will
be aspirated and dispensed repeatedly. “
MIX”
as long as you press the push
button.
4. Set the additional pipetting volume, named
VOLUME 3
: You can choose a
volume to be aspirated and dispensed after the mixing step, so you can continue
routine pipetting.
Press the push button, and then set the volume by turning the push button.
Confirm your settings by a simple click.
5. Fit a tip suitable for the model of PIPETMAN M that you are using (preferably
use PIPETMAN DIAMOND Tips for assurance of accurate and precise results;
refer to Chapter 8
PIPETMAN DIAMOND Tips
on page 13).
Aspirate VOLUME 1
Press the push button.
Dispense
Press and release the push button:
three things happen: sample
dispense, automatic purge and piston
reset to zero. Or, keep your thumb
pressing the push button until the
end of the dispense cycle: the piston
reset takes place after you release the
push button again.
This feature enables you to remove
the tip from the liquid, without
aspirating anything.
Aspirate and Dispense the
Second Volume
“
VOLUME 2
”, repeat steps Aspirate Volume 1 and To dispense.
Mix
Press push button; as long as you keep your thumb pressing the button, the pipette
continues mixing. Release the push button to complete the current mixing cycle.
Purge
Press push button,
PURGE
appears on the screen, click again to purge and reset the
piston.
Pipette
If you have chosen a
VOLUME 3
> 0, you can now aspirate and dispense this volume
by repeating steps 2 and 3.
NOTE
If you set VOLUME 1 or VOLUME 2 = 0 µL, then the step will not appear.
If you set VOLUME 1 and 2 = 0 µL, then the cycle will start directly with
the MIX step.
If you set the VOLUME 3 = 0 µL, then the cycle will stop after the MIX
step.
NOTICE
Always fit a tip before using any pipette.
NOTE
NOTE
If you choose VOLUME 1 = 0, then
step 2 won’t occur, if you choose
VOLUME 2 = 0 µL, then step 4 won’t
occur, as well if you set VOLUME
1 and 2 = 0 µL, then step 1–4 will
disappear. If you set VOLUME 3 =
0 µL step 7, then will not occur.
Figure 4
MIX Mode
START button
Optional STOP
0
V
Purge
Start Purge
Push button pressed
V1
V2
V3
