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PIPETMAN® M
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USER’S GUIDE
4. Fit a tip suitable for the model
of PIPETMAN M that you
are using (preferably use
PIPETMAN DIAMOND Tips
for assurance of accurate and
precise results; refer to Chapter 8
on
page 13).
5. Press push button to aspirate
total volume. The volume
aspirated will be a little bit more
than required (extra volume).
The extra volume is necessary to
ensure equal operating conditions
for each dispensed aliquot.
Dispense
Press the push button.
DISCARD
appears on the screen. Discard the extra volume
andcontinue dispensing the aliquots. PIPETMAN M distributes the aliquot volume each
time you click the push button. The number of aliquots left to dispense is displayed on
the screen. A warning beep indicates the last aliquot.
Purge
Press the push button,
PURGE
appears on the screen, click again to purge and reset
the piston. Keep your thumb pressing the push button until the end of the purge: the
piston reset takes place after you release the push button again. This feature enables
you to remove the tip from the liquid, without aspirating anything.
Eject Tip
Pressing the ejection button.
MIX Mode
This is the classic pipette mode (forward pipetting) followed by a mixing phase, composed
of repeatedly aspirating and dispensing, as well as an optional forward pipetting step.
1.
Press mode button to access menu, where you can choose between different pipette
modes. Select
MIX Mode
by turning the push button, to confirm your selection click
on the push button.
2. Set the first volume, named
VOLUME 1:
press the push button, set the volume by
turning the push button and click to confirm your selection. This is the first volume
you will aspirate and dispense. Set the second volume, named
VOLUME 2
: press the
push button, you can now set the second volume by turning the push button.
Click again to confirm your settings.
NOTICE
Always fit a tip
before using
any pipette.
START button
Optional STOP
V (+ extra vol)*
Purge
Start Purge
V (vol)
0
DISCARD
ALIQUOTS
PURGE
Click to Purge
Discard
Figure 3
REPETITIVE Mode
NOTE
The MIX Mode can be used to prepare a PCR master mix, enzyme restriction
mix, protein solution, oligonucleotide dilution and to mix samples with
gel loading buffer and so on…you can mix two different solutions or
homogenize one solution. If you work with higher viscosity than water (e.g.,
restriction enzyme), you may change the aspiration speed (refer to Chapter 7
on page 13). If you work with genomic DNA, then you
should always pipette very carefully to avoid shearing and nicking.
