System
Compo-
nent
Possible cause
Corrective action
Power
An automatic
circuit breaker has
triggered
•
Turn off the instrument and visually inspect for damage. If
damaged then contact Cytiva.
•
Check if any of the automatic fuses has popped out and push
them back in.
A replaceable fuse
has gone
•
Check the replaceable fuses in the fuse drawer at the power
input.
•
Replace fuse if broken with correct fuse rating see
.
•
Turn on the system and if the problem persists, contact
Cytiva.
Pumps
Pump not working
•
The
Stop button on main screen has been pressed.
•
If problem persists, contact Cytiva.
Little or no flow
•
Check that connected inlet is actually used.
•
Check if liquid is supplied to the system. Check inlet
containers.
•
Check if pressure control valve is open.
•
Check if tubing from inlet container causes pressure or flow
loss. Reasons might be too long tubing, too small internal
diameter, tubing might have a narrow section or is partly
plugged.
•
Peristaltic tubing worn out and flattened or not properly
tensioned inside pump head, re-tension or replace tubing
inside pump head.
Too high feed
outlet pressure
•
Check the outlet to see if the sensor is not plugged.
•
Filter cassette plugged with particulates, replace cassette
and filter feed material with 0.2 micron before performing
protein concentration.
Peristaltic
pumps
Little or no flow
due to broken or
damaged tubing
•
Check if pressure control valve is open.
•
Check the pump tubing.
•
If problem persists, contact Cytiva.
10 Troubleshooting
ÄKTA flux s Operating Instructions 29046893 AG
181
