Step
Action
1
Fill the tank with 0.5 M NaOH.
2
Drain the system.
F – Rinse with 0.5 M NaOH under
pressure
To perform a rinse with 0.5 M NaOH under presssure, follow the steps below
Step
Action
1
Fill the tank with 0.5 M NaOH.
2
If the optional pumps are installed, disconnect the transfer pump tubing
from the transfer pump and the permeate pump tubing from the permeate
pump.
3
Connect the transfer pump tubing (1) with the permeate pump tubing (2).
4
Recirculate the 0.5 M NaOH for 5 minutes by running the feed pump at 75%
of maximum speed. Increase the feed pressure to 2 bar with help of the
retentate and permeate control valves.
5
Drain the system.
G – Recirculate with 0.5 M NaOH for
two hours
To recirculate with 0.5 M NaOH, follow the steps below.
Step
Action
1
Fill the tank with 0.5 M NaOH.
2
If the optional pumps are installed, disconnect the transfer pump tubing
from the transfer pump and the permeate pump tubing from the permeate
pump.
3
Connect the transfer pump tubing (1) with the permeate pump tubing (2).
4
Flush the drain valves with 0.5 M NaOH, use about 50 mL liquid per drain
valve.
5
Fill up the tank with 0.5 M NaOH and recirculate for 2 hours in the system by
running feed pump at 75% of maximum speed. Increase the feed pressure to
2 bar with help of the retentate and permeate control valves.
6
Drain the system.
9 Maintenance
9.2 Sanitization and cleaning
172
ÄKTA flux s Operating Instructions 29046893 AG
