Step
Action
4
Move the tubing connected to the transfer pump outlet, from beaker A to
beaker B (empty beaker), and start to collect flow at the transfer pump
outlet.
5
Collect fluid between time point 0 and time point T. Measure the mass of the
fluid in beaker B with an external balance and calculate the flow rate (weight/
time).
6 Run preparations
6.4 Calibration
6.4.2 Calibration of the transfer pump
116
ÄKTA flux s Operating Instructions 29046893 AG
