BIO RAD ZE5, User Manual

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Chapter 7 Creating Experiments and Workspaces
n After sample boost, the software runs an additional 5 µl of sample to stabilize flow before
acquisition begins.
n
The probe remains in the sample while acquiring the target volume.
n Return Sample is automatically activated. Excess sample in the line returns to the tube or well
after target volume is acquired.
Important: Users can set Return Sample to OFF if necessary. However, when using
Volumetric Counting with Return Sample set to OFF, users will experience sample loss. If
sample loss is a concern, run Volumetric Counting with Return Sample set to ON.
Return Sample
When Return Sample is activated, any sample remaining in the sample line after the required number
of events has been acquired is returned to the tube/well.
Flow Rate or Event Rate Settings
The flow rate controls allow you to specify a precise target flow rate for each position, in µl/sec.
The following flow rate options are available:
n Low — 0.5 µl/sec (30 µl/min)
n
Normal — (default) 1.0 µl/sec (60 µl/min)
n High — 1.5 µl/sec (90 µl/min)
Note: The flow rates values are not editable.
Alternatively, you can specify a target event rate, in events/sec (e/s).
During acquisition in standard mode, you can adjust the target flow rate manually (on the instrument)
from 0.1 µl/sec up to 3.5 µl/sec. However, you cannot adjust the flow rate during acquisition in high-
throughput mode.
Note: In high-throughput mode, all samples must use the same target flow rate.
196 | ZE5 Cell Analyzer and Everest Software