BIO RAD ZE5, User Manual

Page: 393 / 398

Glossary
acquisition Process in which sample data are gathered from the PMTs, processed, and
sent to and displayed in Everest™ Software.
Acquisition mode
In this mode, samples are processed following the programmed steps in the
run list. This mode is typically used after running samples in Setup mode.
area The area under the curve of the pulse, as analyzed by the electronics.
bandpass filter An optical component, usually placed in front of a detector, that allows
passage of wavelengths of light within a specified range while absorbing the
rest.
compensation A mathematical method used to correct the emission overlap from one
fluorophore into the emission channel of another fluorophore. Compensation
can be applied manually or automatically.
cycle mode Shows a current “snapshot” of data defined within the time (in seconds)
specified by the user. Data are automatically refreshed. Cycle mode is
primarily used while adjusting PMT voltages, regions, and gates in Setup
mode.
dichroic filter An optical component, usually part of the detection path, that allows passage of
a range of wavelengths of light while reflecting the rest. Dichroic filters can
either be longpass or shortpass.
event Any signal pulse with an intensity above the threshold in the trigger parameter
(s). See trigger on page 393 and threshold on page 393 .
event rate The number of independent electronic events above the threshold in the trigger
parameter. The event rate is dictated by the number of particles that pass
through the interrogation point per second; however, it is not always equal to
cells per second — debris, cell clumps, and other material also contribute to
the event rate as they pass through the laser.
experiment Another name for a run list.
EYE See ZE5-EYE on page 394 .
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