BIO RAD ZE5, User Manual

Page: 79 / 398

Experiment Builder
Select high-throughput mode to achieve the highest throughput with minimal time between each
sample. After the probe finishes aspirating sample from a position, any programmed wash or agitation
is performed, then the probe immediately moves to the next sample and begins aspirating. Samples
are delivered to the flow cell at a constant rate and sample boost to the flow cell is not utilized.
High-throughput mode cannot be activated for setup (control) wells. Only sample volume limits (not
event limits or gate limits) can be used in high-throughput mode. The Pause After and Return Sample
options are available only in standard sampling mode.
High-throughput
mode Standard
mode
Number of samples (tubes or wells) in sample line
during run.
Multiple One
Boost performed before sample acquisition? No Yes
Return Sample function allowed? No Yes
Pause (between-samples) function allowed? No Yes
Wash between samples allowed? Yes Yes
Agitation allowed? Yes Yes
Event limit allowed? No Yes
Gate limit allowed? No Yes
Volume limit allowed? Yes Yes
Target flow rate can vary per sample? No Yes
Target flow rate range 0.5–2.5 µl/sec
(30–150 µl/min)
0.1–3.5 µl/sec
(6–210 µl/min)
Table 21. Differences between high-throughput and standard mode
For more information, see Selecting Standard or High-Throughput Acquisition on page 199 , Pausing
after a Tube or Well on page 200 , and Returning Sample to a Tube or Well on page 201 .
User Guide | 77