Appendix B
Example 9-Color Immunophenotyping
Experiment
This section provides an example of how to set up a typical multicolor experiment using the ZE5™
Cell Analyzer and Everest™ Software. It shows how to set up compensation controls using the
compensation template and how to apply auto compensation. The sample experiment uses the
following fluorophores:
n
Alexa Fluor 488
n
Brilliant Ultraviolet (BUV) 395
n
APC-Cy7
n
PE (R-phycoerythrin)
n
PE-Cy7
n
PE-Dazzle 594
n
Brilliant Violet (BV) 421
n
Brilliant Violet (BV) 510
n
Brilliant Violet (BV) 711
Preparing Controls and Samples
First, prepare the compensation controls and samples to be placed in your medium of choice, such as
a 40-tube rack.
1.
Prepare a compensation control for each fluorophore to be used in the experiment.
2.
Prepare an unstained cell sample.
3.
Prepare a fully stained test sample.
User Guide | 359
Summary of Contents for ZE5
Page 1: ...ZE5 Cell Analyzer and Everest Software User Guide Version 2 0 ...
Page 2: ......
Page 3: ...ZE5 Cell Analyzer and Everest Software User Guide Software Version 2 0 ...
Page 14: ...Table of Contents xii ZE5 Cell Analyzer and Everest Software ...
Page 50: ...Chapter 2 Hardware Description 48 ZE5 Cell Analyzer and Everest Software ...
Page 82: ...Chapter 4 Everest Software 80 ZE5 Cell Analyzer and Everest Software ...
Page 84: ...Chapter 4 Everest Software 82 ZE5 Cell Analyzer and Everest Software ...
Page 92: ...Chapter 4 Everest Software 90 ZE5 Cell Analyzer and Everest Software ...
Page 104: ...Chapter 4 Everest Software 102 ZE5 Cell Analyzer and Everest Software ...
Page 109: ...Managing Users 9 Click OK to save the new user account User Guide 107 ...
Page 120: ...Chapter 5 Configuring the System 118 ZE5 Cell Analyzer and Everest Software ...
Page 125: ...Working with Optical Filter Configurations User Guide 123 ...
Page 126: ...Chapter 5 Configuring the System 124 ZE5 Cell Analyzer and Everest Software ...
Page 177: ...Setting Up the Run List User Guide 175 ...
Page 191: ...Setting Up the Run List User Guide 189 ...
Page 276: ...Chapter 7 Creating Experiments and Workspaces 274 ZE5 Cell Analyzer and Everest Software ...
Page 295: ...Setting Up the Experiment 6 Set up samples User Guide 293 ...
Page 333: ...Decontaminating the System User Guide 331 ...
Page 392: ...Appendix F References 390 ZE5 Cell Analyzer and Everest Software ...
Page 397: ......