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1- Introduction
WA R N I N G S / C A U T I O N S relating to the use o f BACTEC MYCO/F
LYTIC culture vials with the BACTEC 9050 instrument:
Biosafety Level 2 practice, containment equipment, and facilities are recom-
mended for preparing acid-fast stains and for culturing clinical specimens.
For activities involving the propagation and manipulation of
Mycobacterium
tuberculosis
o r
Mycobacterium
species grown in culture, Biosafety Level 3
practice, containment equipment,and facilities are required as recommended
by CDC.
Because an inoculated leaking or broken vial may produce an aerosol of
mycobacteria, including M.
tuberculosis
or other bacteria, appropriate han-
dling should be observed.
If an inoculated vial is found to be leaking or is accidentally broken during
collection or transport, use the established procedure in your facility for
dealing with mycobacterial spills. As a minimum, "Universal Precautions"
should be employed.Vial should be discarded in an appropriate manner. In
the rare instance where a vial is found to have leaked contents into the
instrument proper, or if a vial is accidently broken, turn off the instrument
immediately.Vacate the affected area. Contact your facility's Safety or Infec-
tion Control Officer(s). Determine the necessity of turning off or modifying
the settings of the air handling units serving the affected area. Do not return
to the area until any potential aerosols have settled or have been removed
by appropriate ventilation. Becton Dickinson Microbiology Systems should
be notified by calling
1-800-544-7434
in the U.S.A. or the appropriate Becton
Dickinson representative in your area. Guidelines for proper handling of
accidental mycobacterial contamination due to breakage of culture tubes or
broth suspensions have been issued by the CDC.'
If recovery of mycobacteria is intended, CDC-NIH guidelines strongly rec-
ommend that the test instrument be placed in the mycobacteriology labo-
ratory where the additional safety issues that the recovery of mycobacteria
present can be addressed.
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