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Negative Vials.
A. Removal
• O p e n instrument door
• Press the 'Remove Negatives' key.
• L C D display identifies negative vials by position and the vial barcode number is also
displayed.
• Remove the vial from the station and scan the vial barcode.
• Repeat the above steps until all negatives are removed (three beeps indicate the process is
complete).
• Press the 'Exit' key and close door.
J IMITATIONS OF THE PROCEDURE:
Contamination
Care must be taken to prevent contamination of the sample during collection and inoculation
into the Bactec vials. A contaminated sample will give you a positive reading, but this does not
indicate a clinically significant result.
Recovery of SPS Sensitive and Fastidious Organisms from Blood Samples
Because blood can neutralize the toxicity of SPS toward organisms sensitive to SPS (such as
some Neisseria species), the presence of optimum volumes of blood, based on media type,
benefits the recovery of these organisms.
Some fastidious organisms, such as Haemophilus species, require growth factors, such as
NAD, or factor V, which are provided by the blood specimen. I f the blood specimen volume is
3.0 mL or less Bactec Peds Plus, an appropriate supplement may be required for recovery of
these organisms. Bactec Brand FOS (catalog #4402153) or Horse serum and Defibrillated sheep
blood may be used as nutritional supplements.
\me
fleneral Considerations
Optimum
recovery of isolates will be achieved by adding the appropriate volume of blood for
the type of vial inoculated. Use of lower or higher volumes may adversely affect recovery
and/or detection time. Blood may contain antimicrobials or other inhibitors which may slow or
prevent the growth of microorganisms. False negative readings may result when certain
organisms do not produce enough CO, to be detected by the system or if significant growth has
occurred before placing the vial into the system. False positivity may occur when the white
blood cell count is high.
DUALITY CONTROL:
Each case of media has a certificate indicating that the media lot has been tested. However,
the laboratory should also test each shipment of media through use of a positive and negative
vial test. The positive vial should be inoculated with 1.0 ml of a 0.5 MacFarland standard of
either Escherichia coli or Staphylococcus aureus. This vial and an uninoculated vial should be
placed into the machine and tested. The inoculated vial should he detected as positive within 72
hours. The negative control vials should remain negative throughout the entire testing protocol.
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