Agilent Technologies NovoCyte Quanteon, Operator'S Manual, Page: 134 / 148

Troubleshooting
134 NovoCyte Quanteon Flow Cytometer Operator's Guide
NovoFlow container is empty Refill the NovoFlow container following the
procedures described in associated instrument
operator's guide.
NovoFlow container is not
correctly connected to the
instrument and the NovoFlow
solution does not flow through
the tubing Reconnect the NovoFlow quick coupling
connectors to ensure the NovoFlow container is
correctly connected to the instrument.
Execute
Prime function from the NovoExpress
Software.
Sample Injection Probe is
clogged Run NovoClean as sample to remove the things
clogged in SIP.
Flow cell is clogged Execute the Unclog function from the
NovoExpress Software.
Sample tubing is leaking Clean the leaked fluid with wipes and properly
reconnect the sample tubing.
Air bubbles exist in the
Sampling Pump Execute Debubble function from NovoExpress
Software.
High CV Air bubbles exist in the flow
cell Execute the Debubble function from
NovoExpress Software.
Flow cell is clogged Execute the Unclog function from NovoExpress
software
Improper sample preparation Re-prepare the sample properly.
The sample flow rate is set
too high Lower the sample flow rate setting in the
NovoExpress software.
Excessive debris in
display Sheath in-line filter is dirty Replace the sheath in-line filter following the
procedures described in associated instrument
operator's guide.
The threshold is set too low Increase the threshold setting and re-collect the
sample.
Fluidics system tubing is dirty
•
Execute Rinse function from NovoExpress
Software.
•
Execute Cleaning function from
NovoExpress Software.
•
Execute Decontamination function from
NovoExpress Software.
High event rate The threshold is set too low Increase the threshold setting and re-collect the
sample.
Sample concentration is too
high Re-prepare the sample to appropriate
concentration or dilute the sample.
Sample flow rate is too high Reduce the sample flow rate.
NovoExpress
displays
Instrument
Workstation is in hibernation
causing the interruption of
•
Restart NovoExpress Software.
• Restart workstation.