Agilent Technologies NovoCyte Quanteon, Operator'S Manual, Page: 133 / 148

Troubleshooting
NovoCyte Quanteon Flow Cytometer Operator's Guide 133
7 Troubleshooting
This chapter describes general instrument troubleshooting and diagnostic
guidelines.
NOTE
For technical support, please refer to Technical Support in this guide.
Table 11 provides guidance on troubleshooting NovoCyte Quanteon instrument
and associated accessories.
For complete list of NovoExpress software prompted error or warning messages
when operating Agilent flow cytometer and associated accessories, please refer to
NovoExpress Software Guide .
NOTE
Please contact Agilent Technical Support if issues are not resolved after performing
the recommended solutions or if there are any issues which are not listed in these
two tables.
NOTE
Investigate possible causes and perform the recommended solutions in the given
order.
Table 11 Instrument Troubleshooting Guide
Observation Possible Causes Recommended Solutions
Instrument QC test
fails QC particles are expired or not
stored properly Prepare a new tube of QC particles and mix
them well to run the QC test again.
Flow cell is clogged Execute Unclog function from the NovoExpress
software.
Flow cell is dirty Execute Cleaning function from the
NovoExpress software.
Air bubbles exist in the flow
cell Execute Debubble function from the
NovoExpress Software.
Zero or low number
of events detected Threshold is set too high Lower the threshold setting and re-collect the
sample.
Sample concentration is too
low Redo sample preparation using an appropriate
method to concentrate the sample.
Sample did not mix well Mix the sample well to suspend the cells.