Agilent InfinityLab LC Series Vialsamplers User Manual
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Introduction
Operating Principle
Operating Principle
Sampling Sequence
The Vialsampler processor continuously monitors the movements of the
vialsampler components during the sampling sequence. The processor defines
specific time windows and mechanical ranges for each movement. If a specific
step of the sampling sequence can’t be completed successfully, an error
message is generated.
During the sampling sequence, the solvent bypasses the vialsampler via the
injection valve. The gripper arm selects the sample vial, either from a static
sample rack, or from external vial positions. The gripper arm places the sample
vial below the injection needle. The required volume of sample is drawn into the
sample loop by the metering device. Sample is applied to the column when the
injection valve returns to the mainpass (main path) position at the end of the
sampling sequence.
The sampling sequence occurs in the following order:
1
The injection valve switches to the bypass position.
2
The piston of the metering device moves to the initialization position.
3
The gripper arm moves from the home position, and selects the vial. At the
same time, the needle lifts out of the seat.
4
The gripper moves into the needle station and stops in the draw position.
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5
The needle lowers into the vial.
6
The metering device draws the defined sample volume.
7
The needle lifts out of the vial.
8
The gripper arm moves out slightly and stops in the wash position
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.
9
The needle moves downwards and dips into the wash well of the wash port.
Simultaneously the peristaltic pump delivers the flush solvent.
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10
The needle moves back.
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11
The gripper arm moves out of the needle station and the wash port snaps
back in position.
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only if automated needle wash is selected. If this feature is disabled, the gripper arm
positions the sample vial directly below the needle (Step 4) and lowers the needle into
the vial.
