Agilent InfinityLab LC Series Vialsamplers User Manual
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4
Preparing the Module
Setting up the Vialsampler
Table 17
Method parameter settings
Injection
Specify the injection volume (in µL) in the
Injection Volume
field. The injection volume limits depend upon the configu-
ration settings of the autosampler. Note that this setting is
ignored when Pretreatment/Injector Program is defined.
Needle Wash
Mark the
Enable Needle Wash
check box to specify an injec-
tion with needle wash.
The following wash parameters can be specified:
•
Mode
: Select the wash location by clicking the down
arrow and selecting the needle wash mode from the
list. You can select from Flush Port (default location) or
Wash Vial.
•
Time
: This field is enabled only if you have selected
Flush Port. Specify a flush time in seconds between 1
and 100 in steps of 1.
•
Location
: enabled only if you have selected a Wash Vial.
Allows you to specify a wash location in the format Vial
X, where X is the vial number. Specify well locations in
the format P<Plate #>-<Row Character><Column Num-
ber>, for example, P1-A1. The values of <Row Charac-
ter> and <Column Number> depend on the wellplate
type.
•
Repeat
: available only if you have selected Wash Vial.
You can specify up to 10 repeat washes.
Stoptime
: enables you to set the time that the analysis
stops. Limits: 0.01 through 99999 min or
As Pump/No Limit
.
Posttime
: the Vialsampler remains in a post-run state during
the Posttime to delay the start of the next analysis. A Post-
time period can be used to allow the column to equilibrate
after changes in solvent composition (for example after
gradient elution). Limits: 0.01 through 99999 min or
Off
.
