9
Data Analysis
GC Image Basic Information
2D-LC User Guide
274
NOTE
Notice how consecutive fractions of the same first dimension peak have exactly
the same retention time in the second dimension, as they experienced exactly
the same second dimension gradient (in contrast to using continuously shifted
gradients in the second dimension, which leads to consecutive fractions of one
first dimension peak experiencing slightly different second dimension gradients).
But be careful! This is only true if the stepwise shifting of the second dimension
gradients is performed at times, when no peaks are eluting from the first
dimension column.
In case your resulting separation looks different from the one shown above: Your
peaks might show a different first dimension retention time due to the use of
another first dimension pump (in the separation shown above, a binary pump
was used in the first dimension). Check whether the stepwise shifting of the
second dimension gradients was performed at times when peaks eluted from
the first dimension column in your separation and understand the effect this can
have on the second dimension retention times of consecutive fractions of the
same first dimension peak!
