2D-LC User Guide
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Concepts of 2D-LC
Multiple Heart-Cutting and High Resolution Sampling 2D-LC
Peak-based mode in multiple heart-cutting
Figure 5
Peak-based mode
In peak based mode, three parameters determine how peaks are parked:
1
A trigger indicates, if a peak has been detected, e.g. because a reference
signal (if available) exceeds the threshold or the slope as defined in advanced
settings.
2
The cut is parked by switching the valve. This happens either if the peak end is
detected (signal falls below threshold or slope) or if the settable cut size has
been exceeded, whatever comes first. The purpose of the cut size is delaying
the parking such that a defined part of the peak, typically its center, is parked.
3
The width t of the green area, which is used for parking a peak is fix and
calculated from the loop volume V and flow rate F in the first dimension by t =
V/F.
NOTE
Please note that the peak parking may start even before the peak trigger if the
sampling time is shorter than the time corresponding to the loop volume. In this
case, the green area will start left to the trigger triangle.
