8
Run the System
Run the Experiment
2D-LC User Guide
220
Table 29
Recommended conditions in 2D (HPLC) for ASM MHC 2D-LC
Parameter
Value
2D-LC Valve
MHC with 40 µl sample, Transfer Capillary, ASM Factor 3
2
D Column Compartment (MCT)
Column
RRHD Bonus-RP, 2.1x 50 mm, 1.8 µm, 1200 bar (857768-901)
Column temperature
40 °C
Stop time
As pump/No limit
2
D Pump
Heart-Cutting (time or peak based)
Figure 110 Time-based
Figure 111 Peak-based
The Cut-Time (MHC) can vary slightly depending on the configuration and the used hardware.
Mobile Phase A
Water + 0.2 % formic acid
Mobile Phase B
Acetonitrile
Flow Rate
1.0 mL/min
Stop time
ca. 40 min (will not automatically prolonged, if peaks in 2D are not work off)
Post time
6 min
