2D-LC User Guide
211
8
Run the System
Run the Experiment
Table 22
Recommended conditions in 2D (HPLC) for high resolution
Parameter
Value
2D-LC Valve
MHC with 40 µl sample, Transfer Capillary, ASM Factor No
2
D Column Compartment (MCT)
Column
RRHD Bonus-RP, 2.1x 50 mm, 1.8 µm, 1200 bar (857768-901)
Column temperature
40 °C
Stop time
As pump/No limit
2
D Pump
Heart Cutting (time or peak based)
Mobile Phase A
Water + 0.2 % formic acid
Mobile Phase B
Acetonitrile
Flow Rate
1 mL/min
Idle flow
not used
Stop time
40 min (will not automatically prolonged, if peaks in 2D are not work off)
Post time
6 min
Sampling Table
Start 11.15 min, 10 cuts, Cut Size 3.2
Figure 109 HiRes
The Cut-Time (HiRes) can vary slightly depending on the configuration and the used hardware.
2D Gradient:
Analysis 1.25 min, Equilibration 0.50 min
Analytical gradient - Shifted Gradient Shift 1D:
10 % B 0.00 min -
30 % B 20 min
60 % B 1.25 min
Flush gradient
80 % B 0.00 min
+ 2 * column void volume
corresponds approximately to 0.21 min
2
D Detector (DAD)
Diode-array
254 nm, Bandwidth 4 nm
Reference Wavelength 360 nm
Reference Bandwidth
100 nm
Peak width
80 Hz
Stop time
As pump/No limit
