8
Run the System
Configure the 2D-LC System
2D-LC User Guide
196
Configure 2D-LC Hardware
Focus on the 2D-LC Valves and the capillary connection.
1
To find out the correct plumbing of the 2D-LC valve ports, see
2D-LC Valve, Standard (G4236A)”
on page 70, or the 2D-LC online help.
The recommended plumbing of the 2D-LC valve differs between 2D-LC setup
with single loops versus 2D-LC setup with Multi Heart Cutting (MHC) Valves
and concurrent versus countercurrent mode.
Table 12
Hardware setups for 2D-LC modi
2D-LC mode
Hardware setup
Standard heart-cutting
•
2D-LC Valve with one single loop
•
2D-LC Valve with two single loops
•
2D-LC Valve with two MHC valves (each with six Sample Loops)
•
2D-LC ASM Valve with two MHC valves (each with six Sample
Loops)
MHC or HiRes
•
2D-LC Valve with two MHC valves (each with six Sample Loops)
•
2D-LC ASM Valve with two MHC valves (each with six Sample
Loops)
Comprehensive
•
2D-LC Valve with two single loops and 2D-LC Valve with two MHC
valves (each with six Sample Loops)
•
2D-LC ASM Valve with two MHC valves (each with six Sample
Loops)
NOTE
40 µL sample loops are part of the default setup in the methods of the data
media.
NOTE
The 2D-LC valve with one single loop setup is only used for special applications,
for example the Bio ProA-Sec Kit.
For more information, see the bio application documentation.
NOTE
Methods for preparation and checkout runs of recommended system
configurations are available on the Agilent 2D-LC Software data media.
Not all possible configurations can be shown here. Therefore, adapt these
methods for other configurations and modules if necessary.
