2D-LC User Guide
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8
Run the System
Run the Experiment
Run the Checkout Familiarization Procedure for ASM
Multiple Heart-Cutting (MHC)
To run the checkout, various hardware configurations are possible, see
on page 56. Not all options can be shown. As example the
is used here.
The following parameters have been optimized for this standard configuration.
Parameters can deviate slightly for your system. Run the experiment and
subsequently edit or optimize the method for your setup.
Table 28
Recommended conditions in 1D (HPLC), ASM MHC
Parameter
Value
1
D Column Compartment (MCT)
Column
RRHD SB-C18, 2.1x 100 mm, 1.8 µm, 1200 bar (858700-902)
Column temperature
40 °C
Stop time
As pump/No limit
1
D Pump
Mobile Phase A
Water + 0.2 % formic acid
Mobile Phase B
Methanol
Flow Rate
0.6 mL/min
Stop time
40 min
Post time
6 min
Mobile Phase
Gradient:
45 % B 0.00 min
54 % B 6.00 min
80 % B 7.00 min
Autosampler
Injection Volume
2 µLfor Standard LC
0.5 µL Positive Mode for LCMS
10 µL Negative Mode for LCMS
Injection Needle Wash In Flush Port, 10 s, acetonitrile/water (50/50)
Stop time
As pump/No limit
1
D Detector (DAD)
Diode-array Detector
Signal A
254 nm, Bandwidth 4 nm
Reference Wavelength 360 nm
Reference Bandwidth
100 nm
Peak width
20 Hz
Stop time
Stop time As pump/No limit
