2D-LC User Guide
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2D-LC Data Acquisition in MassHunter Workstation 11
2D-LC User Interface
Prepare Pump
Allows you to control the Purge, Condition, or the Prime function.
•
Purge:
Purge the LC pump. Fill the system with fresh or different solvent.
Follow the directions for purging the pump in the user guide for your
pump.
•
Conditioning:
Condition or equilibrate the column. After you purge the pump, you set
up to condition or equilibrate the column.
a
Enter LC parameters in the Method Editor menu, and click Apply to
download the method parameter to the LC or,
b
To select an LC conditioning method, select one from the Method
list at the top of the Data Acquisition window.
NOTE
Conditioning can also be used to remove micro air bubbles. For
this measure you have to use a reasonable flow rate (for example
1.5 mL/min), composition setting (for example A: 50 % B: 50 %)
and backpressure (
>
200 bar) to ensure efficient air bubble
removal from all pump heads. For further info, please follow the
instruction in the technical note
Best Practices for Using an Agilent
LC System
.
•
Prime:
If conditioning for 15 min cannot remove air from the pump heads, the
Prime function can help. The module draws 20 times solvent at a high
speed with all pump drives simultaneously and dispenses it into the
waste position of the automatic purge valve. The Prime function
stresses the valve and rotor seal. Therefore, it should be performed
only as a last measure, before forcefully filling the pump heads with a
syringe or attempting to repair the pump heads.
Flush sample loops
Use the gradient start condition for flushing all 2D sample loops and flush
the 2D flow path with the flush gradient defined in the method.
Seal Wash Prime
Allows you to refill the Seal Wash lines once the seal wash solvent has
been changed.
Switch Valve
Allows the selection of different valves e.g. ASM Valve and the change of
their valve position
Modify
Allows you to configure/modify the 2D-LC capillaries and the transfer
volume.
Modify Capillaries
Displays the Modify Capillaries dialog box. In this window you can
configure the sample loop, transfer capillary and ASM capillary, see
