Waters ACQUITY UPC2, Обзор и руководство по обслуживанию, Страница: 37 / 84

Using a cuvette 33
3. With the spring guide facing you, gently insert the cuvette (containing
eluent) up and under the guide, with the cap facing upward (into the
holder) and a frosted side of the cuvette facing up. Refer to the figure on
page 19 .
Recommendations:
• Ensure that you have enough liquid (3 mL) in the cuvette so that,
when it is inserted into the holder, you can see liquid through the
cuvette holder aperture (that is, the liquid completely covers the
aperture).
• Because the cuvette holder is angled, use your thumb or forefinger
to ensure the cuvette is secure in the slot and does not slide forward.
• Ensure that it does not become dislodged when you are replacing
the cuvette holder.
4. Gently guide the cuvette holder back into the flow cell assembly until it
sits in position securely.
To prevent invalid subsequent chromatographic results, remove the
cuvette from the detector, and replace the empty holder after running
your cuvette measurements.
5. Close the detector door.
Recommendation:
To maintain optimum system and noise
performance, ensure the detector door is fully closed before resuming
normal operation of the detector.
6. Insert a reference cuvette containing the diluent standard, and run a
zero measurement.
7. Replace the reference cuvette with a cuvette containing your analyte in
diluent, and run a sample measurement.
8. Use the storage, review, subtract and review, and replay functions to
analyze the data obtained.