Waters ACQUITY UPC2, Обзор и руководство по обслуживанию, Страница: 36 / 84

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Restriction:
Because the measurement is actually a composite of both the
contents of the cuvette and the flow cell, you need to perform cuvette
measurements under identical flow cell conditions. If you store spectra and
acquire new spectra for subtraction, you need to be aware of differences, if
any, in flow cell conditions.
Ideally, you should perform both the zero and sample measurements using the
cuvette when the instruments are in the idle or static state and operating
under identical flow cell conditions.
Before you begin
To ensure accurate results, use a 10-mm path length quartz cuvette and
matched pairs (from the same manufacturing lot) of quartz cuvettes for your
zero and sample measurements.
Before beginning a measurement using the cuvette, fill the flow cell by
flushing with 10 mL of the same diluent that you are going to use with the
cuvette measurement. Then, to ensure a clear and clean cuvette, wipe the
clear portion of the cuvette with low-lint, nonabrasive tissue wipes.
Cuvette measuring procedure
To begin a cuvette measurement:
1. Open the detector door.
2. To remove the cuvette holder, slide it toward you.
Caution:
Handle the cuvette gently and on the frosted sides only.
Fingerprints on the clear quartz interfere with the light path and
compromise the integrity of cuvette measuring operations.
Cuvette holder
Flow cell
identification
cable