UVITEC Cambridge FIREREADER MAX, Руководство пользователя и обслуживания

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UVITEC – Cambridge 6/225
EXPOSURE MENU ......................................................................................................................................................44
Access to the Exposure modules ............................................................................................................................... 44
Auto-exposure ........................................................................................................................................................... 46
Manual exposure ...................................................................................................................................................... 49
Serial exposure .......................................................................................................................................................... 52
Focus point adjustment and motorized zoom controls ............................................................................................. 54
ANALYSIS MENU ........................................................................................................................................................56
Access to the analysis modules ................................................................................................................................. 56
M
OLECULAR WEIGHT ANALYSIS MODULE
..........................................................................................................................58
Objectives and output
......................................................................................................................................................58
Molecular weight module (MW) operating environment
........................................................................................59
Toolbar in details
..................................................................................................................................................................62
1 - D
E T E C T
............................................................................................................................................................. 69
A – Edit lane
..........................................................................................................................................................................69
B – Edit band
.........................................................................................................................................................................73
C – Add marker values
......................................................................................................................................................77
2 - A
N A L Y S E
– M
O L E C U L A R W E I G H T
........................................................................................................... 82
A – Molecular weight results
.............................................................................................................................................82
Return to the 1D acquisition module ........................................................................................................................ 83
C
OLONY COUNTING ANALYSIS MODULE
............................................................................................................................84
Objectives and output
......................................................................................................................................................84
Colony counting (CC) module operating environment
..........................................................................................85
Toolbar in details
..................................................................................................................................................................87
A
U T O M A T I C C O U N T I N G
.................................................................................................................................... 94
Detection
..............................................................................................................................................................................94
Return to the 1D acquisition module ........................................................................................................................ 95
M
A N U A L C O U N T I N G
........................................................................................................................................... 96
Manual counting
.................................................................................................................................................................96
Return to the 1D acquisition module ........................................................................................................................ 97
D
ISTANCE CALCULATION MODULE
...................................................................................................................................98
Objectives and output
......................................................................................................................................................98
Distance calculation module operating environment
.............................................................................................99
Toolbar in details
................................................................................................................................................................101
1- D
E T E C T
......................................................................................................................................................... 108
A – Edit lane
........................................................................................................................................................................108
B – Edit band
.......................................................................................................................................................................111
C – Distance
.......................................................................................................................................................................114
2 - A
N A L Y S E
– D
I S T A N C E
............................................................................................................................... 117
A – Distance results
...........................................................................................................................................................117
Return to the 1D acquisition module ...................................................................................................................... 118
O
PTICAL DENSITY
1D
ANALYSIS MODULE
........................................................................................................................ 119
Objectives and output
....................................................................................................................................................119
Optical density / 1D (OD-1D) operating environment
............................................................................................119
Toolbar in details
................................................................................................................................................................122
1- W
I N D O W D E F I N I T I O N
.............................................................................................................................. 128
A – Lane definition
............................................................................................................................................................128
B – Background subtraction
...........................................................................................................................................131
C –Spot separation
...........................................................................................................................................................136
2- A
N A L Y S E
– S
P O T Q U A N T I F I C A T I O N
................................................................................................... 140
Principles of quantification
.............................................................................................................................................140
A –Quantity of reference
................................................................................................................................................141
Return to the 1D acquisition module
...........................................................................................................................144
EDIT MENU .............................................................................................................................................................. 145
Access to the Edit module ....................................................................................................................................... 145
Text ......................................................................................................................................................................... 146
Crop ......................................................................................................................................................................... 150
Rotation .................................................................................................................................................................. 151
Pseudo color ............................................................................................................................................................ 152