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Thermo Fisher Scientific

User Guide STP420D/STP420ES

routine operations

Editing and Creating Protocols

Note!

Copying a protocol: You can also select Copy to copy an existing protocol,

then use Edit to modify it.

Note!

Deleting a protocol: You can delete a protocol by selecting Delete.

Note!

Changing protocol steps: If a protocol is running, you can modify parameters

for steps that have not been processed yet. For example, you can change para-

meters for step 3 and the following steps while running step 2. However, you

cannot insert or delete protocol steps, and you cannot change the current step.

Note!

Protocol changes: Changes made to a running protocol are not stored

permanently. They are only valid for the current protocol run.

opyinG

protoCol

If you have copied a protocol, rename it.

Содержание STP420D

Страница 1: ...1 Thermo Fisher Scientific User Guide STP420D STP420ES Part of Thermo Fisher Scientific STP420D STP420ES Thermo Scientific Tissue Processor Instruction manual English 387791 Issue 1 1 ...

Страница 2: ...02 Thermo Fisher Scientific User Guide STP420D STP420ES ...

Страница 3: ...ermo Fisher Scientific User Guide STP420D STP420ES STP420D STP420ES Thermo Scientific Tissue Processor Instruction manual English 387791 Issue 1 1 Figure 1 Thermo Scientific Microm STP420ES Tissue Processor ...

Страница 4: ...with Thermo Fisher Scientific or your local Thermo Fisher Scientific representative This User Guide may not in whole or in part be copied photocopied reproduced translated or converted to any electronic or machine readable form without prior written consent of Thermo Fisher Scientific All information included in this User Guide is proprietary and confidential and the exclusive property of Thermo F...

Страница 5: ...tion for example unshielded intentional RF sources as these may interfere with the proper operation The electromagnetic environment must be assessed prior to the operation of the device This User Guide covers both models of the Thermo Scientific Microm STP420 Tissue Processor the STP420D produced between 2006 and 2009 and the STP420ES produced from February 2010 onward The Thermo Scientific Microm...

Страница 6: ...es at the nearest Thermo Scientific sales office by giving the serial number of the instrument the number of the User Guide and the date of issue This User Guide is available in the following languages Cat No German 387790 English 387791 French 387792 Spanish 387793 Italian 387893 Please check the serial number on the type plate which is placed on the left side panel of your instrument and enter t...

Страница 7: ...orkmanship for a period of 1 year Parts which prove to be defective during the warranty period will be repaired or replaced free of charge by Thermo Scientific No other warranty is expressed or implied Unauthorized modification or repair by third party persons will void the warranty The warranty will expire in case of improper or wrong use of the instrument and in case the warning and precautionar...

Страница 8: ...operation of the instrument Warning or caution Warning or Caution Special precautionary measures to prevent damage to the equipment Observe these carefully to prolong the life of the instrument General Danger Observe the Us er Guide strictly when this symbol is visible Never override the safety features built in to the instrument Biohazard There is potential danger from biological material Chemica...

Страница 9: ...hree wire ground plug The power outlet must be con nected to the protective earth and must meet the Internation al Electrotechnical Commission IEC regulations Do not cut deform or remove any of the prongs from the power cord You can not operate this device using an extension cord Mains Voltage Never remove the instrument covers during operation Only trained service personnel may replace components...

Страница 10: ... observe the general laboratory regulations concerning protection against danger of infection You can find information on decontamination media and their use dilution and effective range of application in the Laboratory Biosafety Manual 1984 of the World Health Organisation Unhealthy or irritating substances Small amounts of irritating aerosols are possible during the intended use of the instrumen...

Страница 11: ...our country and Thermo Fisher products which might help in the detection of substances subject to the RoHS directive at www thermo com WEEERoHS The User Guide uses the following conventions Touch screen buttons are designated in bold for example Select Reagents If you are not sure what the button looks like see Section 1 5 1 Buttons The User Guide uses a number of Note and Warning symbols to draw ...

Страница 12: ...ns such as language reagent rotation and so on Routine Operations This chapter tells you how to perform routine tasks not covered in Chapter 1 or Chapter 2 including how to empty containers replace filters manage and replace reagents create and edit protocols and so on Troubleshooting and Errors This chapter describes some common easily resolved problems error messages and codes It does not cover ...

Страница 13: ...20 38 2 2 Selecting a Site for the STP420 39 2 3 Transporting the STP420 to Its Site 41 2 3 1 Moving the STP420 using rollers 42 2 3 2 Carrying the STP420 using the transport handles 43 2 3 3 Moving the STP420 using lifting equipment 44 2 4 Preparing the STP420 and Turning It on 45 2 4 1 Turning on the power 45 2 4 2 Filling the reagent bottles manually 46 2 4 3 Filling the paraffin containers 47 ...

Страница 14: ... Protocol 70 3 5 1 Running a protocol 70 3 5 2 Adding more specimens while a protocol is running 73 3 6 Cleaning Baskets and Replacing Reagents 74 3 6 1 Running a cleaning cycle 74 3 6 2 Manually replacing reagents 75 3 6 3 Automatically replacing reagents 76 3 7 Editing and Creating Protocols 80 3 7 1 Editing a protocol 80 3 7 2 Protocol steps and options 83 3 7 3 Creating a new protocol 84 3 8 E...

Страница 15: ...ces of the Instrument 106 5 5 Routine Maintenance 107 5 6 Service Contract 107 Transportation and Final Shutdown 108 6 1 Transportation Preparing for Shipment 108 6 2 Final Shutdown 110 Appendix 111 Standard Equipment 111 User and Supervisor Permissions 112 Users 112 Supervisors 113 Factory Default Settings 114 Approved Reagents 115 Technical Data 116 Preferred Protocols and Best Practices 118 Pro...

Страница 16: ...samples for laboratory testing by fixing dehydrating clearing and infiltrating them with paraffin Processing is governed by protocols and reagent programs which you create and edit 1 1 Overview Introduction Overview Figure 2a General layout of the instrument 1 Touch screen 2 Paraffin reservoir 3 Rotary chamber 4 Biopsy chamber 5 Reagent Managment Panel 6 Reagent stations 1 2 3 4 5 6 ...

Страница 17: ...rked safety measures regulations and hygiene measures of your laboratory Figure 2b General layout of the instrument 1 Interfaces type I covered 2 Touch screen moveable arm 3 Toolbox Optional interfaces 4 Interfaces type II 5 Serial number plate 6 Main switch Interfaces The STP420 has a console interface which is located either on the left side of the device 4 or on the left side of the monitor 1 2...

Страница 18: ...er System A versatile protocol system see Section 1 10 Protocols You can define tissue processing protocols exactly suited to the needs of your laboratories and any special quality controls You can adjust these protocols easily if your laboratory requirements change The processing protocols are stored on an integrated hard disk The number of protocols you can store depends on the number of steps i...

Страница 19: ...return the system goes into emergency mode The chamber continues to rotate for sixty minutes Duration 5 minutes when paraffin has been filled If power is then still not available the instrument goes into shut down mode with the tissues safely stored in reagent Protection features and sophisticated software controls to guarantee that in the event of a power failure or other problems during the infi...

Страница 20: ...rd Not necessary but can be connected to the PS 2 interface for service purposes RJ45 LAN To establish a connection to a service technician s laptop Two USB ports For storing protocols on the USB data stick provided Note USB data sticks Only use the USB data stick provided others will not work If the USB data stick provided does not work initially shut down the STP420 Section 3 1 2 Turning the ins...

Страница 21: ...onnecting the keyboard or mouse for maintenance purposes or for operating the device In addition the connectors can be used to transfer logs on a USB memory stick 2 LAN to establish a connection to the laptop of a customer service representative 3 COM1 for remote diagnostics and remote access to each function 4 VGA for connecting an additional monitor 1 2 3 4 ...

Страница 22: ... cassettes must be removed from the holder individually The basket organisers giving a loading capacity of 33 cassettes divide the basket space into 33 individual compartments In this configuration the Cassettes are always held vertically with the front or rear accessible You can load up to six holders in the rotating section and one holder in the biopsy chamber for one run Processing starts after...

Страница 23: ...within a few minutes An integrated screen saver turns off the monitor when not in use This extends the length of the screen life In order to turn the monitor on again touch the screen When you first turn on the equipment the touch screen monitor displays the Standby screen On the right at the top of the screen is the Status window For details of the Status window see Section 1 5 3 Status window 1 ...

Страница 24: ...the Status window are seven buttons for the main functions of the instrument As processing continues different command buttons see Section 1 5 1 Buttons appear at the bottom of the screen for example Figure 6 Example for different buttons taking the place of the general message line during processing Import Help Edit New Copy Delete Start Figure 5 General message line on the bottom of the screen 1...

Страница 25: ...g protocol so that you can edit it to create a new one Delete Press to delete a programm Delete Reagent This button lights up after selecting reagents as a part of the reagent management if a reagent station is highlighted on the screen Pressing this button deletes the previously assigned reagent from that station Diagnosis This gives you access to all the message protocols message codes and hardw...

Страница 26: ...Import Press this to retrieve a protocol from a floppy disk or USB data stick Language Press this button to choose a language The default is English List Press this while a protocol is running to see a list of predefined protocols Lock Press this button to change the screen status to Locked Manual Use this button on the Reagent management screen if you want to redefine reagents New Use this to cre...

Страница 27: ...iety of adjustable settings such as user level language date and time volume temperature units quick start buttons and process settings Shutdown Press this button to shut down the instrument correctly before turning the power off Start Press this button to start a predefined protocol Table Press this to display protocol progress as a table User This button allows change the user level Station clea...

Страница 28: ...irectly below it Maximum and minimum possible values for the parameter appear immediately below the input field The instrument checks that the value you enter is valid Confirm your entry by pressing Enter Note Invalid entries Entries that fall outside the valid limits are shown in red and cannot be confirmed by pressing Enter In this case use the key to delete digits Figure 7 The numeric keypad 1 ...

Страница 29: ...ames reagent names and passwords Note Password security For security as you type the password in the input field displays asterisks rather than your typed characters Note Upper case Press Shift for upper case letters Upper case remains active until you press Shift again Figure 8 The alpha numeric keypad Introduction Touch Screen Monitor ...

Страница 30: ... displays warning messages and an audible warning sound can be heard Press the alarm bell icon to turn this off Melting point timer This icon is visible while the paraffin is melting While it is displayed paraffin is not available for processing tissue specimens As soon as the paraffin is ready the icon disappears Note Molten paraffin You can add molten paraffin to speed up the melting process The...

Страница 31: ...program is running for example to add more specimen The supervisor is free to pass this option for the User level For a comprehensive list of the tasks that supervisors can perform see Appendix B User and Supervisor Permissions Demo This mode simulates processing and is for demonstration purposes only for example at trade fairs and so on Technician This level has special protection To access it yo...

Страница 32: ...the line in order to use the two chambers simultaneously Heating is applied to the containers from below and via a heating core in the middle of each container The initial paraffin melts with the entire volume melting in approximately nine hours Subsequently melting takes approximately four hours You can clean the drain mesh or remove objects that fall into the tank when you lift the heat distribu...

Страница 33: ...to the ambient air It is pumped through the chamber and separated from the solvent vapors in two steps The pumped air is guided through the wash bottle which is filled with water The water soluble constituents of the vapors for example alcohol are completely condensed in this bottle Then the air is passed through the large active charcoal filter which filters any vapor still remaining Note idle ti...

Страница 34: ...ke sure you observe strictly the following replacement intervals Wash bottle After five program runs or once a week see Section 3 3 Filter System Maintenance Active charcoal filter After approximately six months see Section 3 3 Reagent filter twice a week see Section 5 3 Rotary and Biopsy Chamber Cleaning Cylce ...

Страница 35: ...n reagent stations and two cleaning stations see Section 3 4 Assigning Reagents and Paraffins to Stations Follow these rules when using reagents Handling reagents Handle the reagents used with the instrument according to your laboratory s regulations Transporting reagents Carry the reagents in the reagent bottles in such a way as to avoid spilling any reagent Storing reagents Store reagents in an ...

Страница 36: ...of possible protocols and protocol steps you can store is limited only by the storage capacity of the built in hard disk Protocols are normally designed to run only for the defined processing time plus the necessary additional times for filling and draining reagents in the chambers heating and pressure build up However specimen processing is usually carried out over night In these cases you are ad...

Страница 37: ... 2 Selecting a Site for the STP420 3 Transport the STP420 to its correct location see Section 2 3 Transporting the STP420 to Its Site 4 Prepare the equipment and turn it on see Section 2 4 Preparing the STP420 and Turning It on 5 Set the various processing options such as language date and time user level and so on see Section 2 5 Setting the Processing Options 6 Create an initial reagent list see...

Страница 38: ... of Thermo Scientific All warranties and guarantees are null and void if the instrument is installed by a third party To unpack the STP420 1 Open the packaging by following exactly the instructions affixed to the surface of the transport packing 2 After unpacking store all the packing materials that is the cardboard box wooden pallet polystyrene plastic bag and so on in a dry condition 3 In the ev...

Страница 39: ... does not need a fan or exhaust vent for safe operation However you can connect a 50 mm diameter hose for external fume extraction if required The socket on the rear has an outer diameter of about 51 5 mm The hose NW 50 e g HT connection hose DN 50 needs to be attached to the socket It can be connected to the downstream suction connection of a fume hood or an exhaust system Description of the air ...

Страница 40: ... opening the lid of the rotational chamber if it contains formaldeyhyde to avoid exposure To guarantee sufficient ventilation make sure the instrument can be sited the following distances from walls or other instruments Rear 30 cm 12 inches Left side 30 cm 12 inches Right side 20 cm 8 inches All interfaces for peripherals for example remote alarm system are located on the left side of the device T...

Страница 41: ...transporting the instrument make sure no pressure is applied to the side walls or to the front or rear of the instrument Carrying the STP420 using lifting equipment If necessary use more than one person to make sure the instrument does not tilt to the right or left You can move the instrument On rollers Using the transport handles Using lifting equipment These are described in the following three ...

Страница 42: ...oving the equipment over an even floor The STP420 has four rollers suitable for moving the equipment over an even floor see Fig 10 Keep the instrument upright and push it holding the right and left sides Never move the device by holding the locking lever of the biopsy chamber or the handle on the rotating chamber lid Otherwise the locking mechanism may be damaged ...

Страница 43: ... transport handles to the rear wall of the instrument see Fig 11 2 If the lower transport handles are not attached push them into the receptacles and secure them using the clip connectors Clip Connectors Ensure that the clip connectors lock correctly in place 3 Lift and carry the instrument using the upper and lower transport handles Figure 11 Position of the transport handles 1 Transport handle F...

Страница 44: ...420 using lifting equipment When transporting the instrument using a lift truck or a fork lift first completely repack the instrument following the Unpacking Instructions found on the shipping container Carrying using lifting equipment If necessary use more than one person to make sure the instrument does not tilt to the right or left ...

Страница 45: ...must 1 Fill the reagent bottles with reagents see Section 2 4 2 Filling the reagent bottles manually 2 Turn the equipment on see Section 2 4 1 Turning on the power 3 Fill the paraffin containers with pellets flakes or liquid paraffin see Section 2 4 3 Filling the paraffin containers 4 Fill the wash bottle with distilled water see Section 2 4 4 Filling the wash bottle 1 Plug the equipment in 2 Turn...

Страница 46: ...wer line If you intend operating with two chambers simultaneously you must fill the bottles to the upper line Filling the bottles Make sure you keep to the prescribed filling levels when filling bottles with fresh reagent 3 Use the metal tags provided or label the bottle clip with the name of the reagent and insert the filled bottle into its correct location Make sure you hear the click that confi...

Страница 47: ...is time the melting timer will be running A protocol can be started only after the melting timer set off or was turned off You can achieve shorter melt times by using flakes or liquid paraffin If liquid paraffin is used for filling the melting timer can be turned off Wait at least 30 min after switching on the timer before you start the first protocol to ensure that all pipes and valves have reach...

Страница 48: ...n reset the melting timer Press the melting timer icon and Delete Turn off 5 Now go to Section 2 4 4 Filling the wash bottle Note Water level You can see the filling height of the water inside the bottle in the oval window on the right side of the instrument If you have access to the rear of the instrument shining a flash light through the rear panel can help you determine the water level more eas...

Страница 49: ...the 5th message a reminder will be displayed Check the level regularly The maximum mark on the window must not be exceeded See Section 3 3 2 Emptying water from the wash bottle By pressing the button Remind Later the reminder can be postponed as often as it is necessary It appears however after the log of each new message until the counter is reset with OK Note You can reset the counter only when ...

Страница 50: ...en you can Shut the system down properly see Section 3 1 Turning the Instrument On and Off Select the language see Section 2 5 1 Selecting the language Set local time see Section 2 5 2 Setting local time real time clock Adjust the audible volume of signals see Section 2 5 3 Adjusting the audible volume Set the pressure units see Section 2 5 4 Selecting the pressure units Set the temperature units ...

Страница 51: ...o change this You can choose to operate the instrument in English German French Italian or Spanish To change the language 1 On the Process settings screen select Language to display the Standby screen 2 Select the language you want Thereafter all indications and messages on the screen appear in the language of your choice You do not need to restart the instrument Note Changed language It takes app...

Страница 52: ...ease the volume move the slider to the left To increase the volume move the slider to the right To turn the volume off move the slider as far left as it will go You can choose between pounds per square inch PSI and bar millibar bar mbar 1 On the Process settings screen select Language to display the Standby screen 2 Select Pressure in psi or Pressure in bar as appropriate The button legend indicat...

Страница 53: ...creen touch the value box next to the paraffin temperature b Use the virtual numeric keyboard to enter the value you want For example Note Maximum and minimum temperatures The maximum and minimum paraffin temperatures are default settings you cannot change 2 If necessary adjust the paraffin regeneration settings according to your laboratory s specific requirements a At the upper right of the Proce...

Страница 54: ...cates the sytem in use Note For devices with monitor interfaces can print data as pdf only and save them on USB If you print to PDF the PDF files will be stored automatically to the selected I O device External UPS Turn this option on when the STP420 is connected to an external uninterrupted power supply USB data sticks Use only the USB memory stick supplied with the STP420 by inserting into the l...

Страница 55: ...group name d On the keypad select to erase characters or Enter to confirm the name 3 To change the color associated with a reagent displayed on the screen a Under Reagent group check the group you want to select it b Select a color from the display under Colors in the middle of the screen 4 To switch on or off the audible signal which tells you when an automatic fill or drain has finished select C...

Страница 56: ... the time frame Set ext Alarm A screen for setting the time frame will appear Note Setting the frame only if you have Supervisor access 3 Press the hour or minute field for the start time and enter the desired time using the virtual keypad Follow the same procedure for setting the end time Note You must use a 24 hour clock Factory setting is from 00 00 to 00 00 meaning that the remote alarm is alw...

Страница 57: ... lower part of the screen The Reagent setup screen appears showing a predefined list of reagents you can use for protocols Use the buttons at the bottom of the screen to edit the list if required as shown below Note List editing For safety reasons you can only change the list when no protocol is running Functions which are not yet available are shown in gray You can only edit a list if you have su...

Страница 58: ...gle protocol use reagents that are incompatible with each other such as water and paraffin and issue a signal Note Even when using compatible reagents the device might not issue a warning message if the tissue samples are damaged Note that when defining the protocols always use the General Procedures for Tissue Embedding and tissue specific reagent compatibility Use the reagent category dehydratio...

Страница 59: ...e In the case of protocols running these reagents the chamber will be emptied brfore the lid is unlocked 7 Touch the value boxes next to each reagent management property to assign values using the virtual keyboard 8 Touch Enter 9 Touch OK to save the changes All used reagents must be allocated to a reagent category Using these categories the STP 420 can predict if the sequential steps in a single ...

Страница 60: ...only save the reagent list if you have supervisor access Save the list of defined reagents to the USB data stick or 3 5 inch floppy disk as a backup 1 To export the list of defined reagents either insert an MS DOS formatted floppy disk into the floppy disk drive or a USB data stick into an available USB ports 2 Select Export in the lower part of the screen The list is stored on the floppy disk or ...

Страница 61: ...sed by not turning the instrument off properly is excluded from the warranty and any repair and or service call will be charged To turn the instrument off correctly 1 First Press the button Shutdown in the bottom right of the monitor and confirm with Yes 2 The device shuts down At the end of this process is on the screen Shutdown Complete Among them appear the lines 558 469503 SysRq Emergency Sync...

Страница 62: ...rs is described in Section 2 4 3 Filling the paraffin containers in Chapter 2 Setting Up the STP420 Empty the paraffin tank with the help of the connection console on the front panel over the reagent station 1 Open the glass door for access see Fig 23 A 2 Tighten the connection rail by pulling the tension rod down and out completely see Fig 23 B 3 Open the protective cover see Fig 24 A 4 Place a c...

Страница 63: ...araffin container 7 Lock the paraffin tab closed again see Fig 25 Let the paraffin drip for a short time so there is no paraffin left in the drain 8 Push the rail back into the device 9 Close the protective cover Routine Operations Filling and Emptying the Paraffin Containers Figure 25 Close the paraffin tab Figure 24 Open the paraffin tab C B A ...

Страница 64: ...he rear cover and remove the wash bottle screw cap see Fig 26 A 2 Open the glass door of the reagent cabinet 3 Place an empty container with a liquid capacity of approximately 2 liters in front of the instrument 4 Insert the drain hose into the container You can identify the drain hose by its color It is the same color as the H2O connector on the device see Figure 26 B 5 Attach the other end of th...

Страница 65: ... you connect to the instrument 6 Let the used filter water drain into the container until the wash bottle is completely empty 7 Detach the drain hose by pressing the metal piece downward on the connection and pulling the hose off The connection automatically closes 8 Dispose of the used filter water according to your laboratory s regulations 9 Refill the wash bottle with distilled water see Sectio...

Страница 66: ... the Remind Later button The prompt will appear again before the next protocol is started and can be deferred or acknowledged with OK Note You can reset the counter only when you have Supervisor access 1 Open the cover behind the paraffin containers with an upward movement 2 Firmly pull up the filter cartridge 3 Insert a new filter cartridge making sure it locks correctly into place To replace the...

Страница 67: ...Note Assigning reagents You can only assign reagents if there is no protocol running 1 Select Reagents on the Standby screen The Reagent management screen appears 2 Select the reagent station you want on the touch screen to activate it The station selected is marked with a frame for example Routine Operations Assigning Reagents and Paraffins to Stations 3 4 Assigning Reagents and Paraffins to Stat...

Страница 68: ... to the selected station 5 You can now fill the station bottle with the selected reagent in one of two ways Manually By refilling the reagent bottle Automatically Using the automatic filling function see Section 3 6 3 Automatically replacing reagents Note If the desired reagent station has a reagent already assigned pressing the Manual button will assign it as an empty bottle pressing Delete will ...

Страница 69: ...the lower part of the screen change to display the available functions These are Regeneration The regeneration function is designed to work when isopropanol not ethanol or Xylene is used for dehydration and clearing If you select Regenerate a special protocol starts in the rotary chamber All the paraffin in the container is treated at an increased temperature and with a vacuum This evaporates resi...

Страница 70: ...mes 3 In case a wait step is already defined in the protocol you can select No to change the defined fixed end of the protocol This corresponds to the definition of a fixed end time of a protocol the protocol will remain longer in the predefined wait step The No button appears only if a wait step was predefined in the protocol Select Yes to go ahead and start the protocol Note Start time If the st...

Страница 71: ... a protocol Note Editing protocols You can only edit a protocol if you have supervisor access Press Open to open the chamber If you want to add more specimens while a protocol is running see Section 3 5 2 Adding more specimens while a protocol is running Note Opening and cancelling If you select Open or Cancel the screen asks a number of user questions before proceeding If the reagent is marked as...

Страница 72: ...ic or as a table From the start the device performs a protocol of various self tests including a pressure test When the chamber is not closed properly the screen displays an error code When the preparatory tests are completed successfully the protocol first step starts To prevent misuse of the device the touch screen is locked and will show the Locked icon see Section 1 6 User Profiles in the Stat...

Страница 73: ...ins the reagent before the lid is released when you try to open the chamber 4 Press OK in response to Rotation biopsy chamber lid is unlocked and can be opened 5 Open the lid add the cassettes and close the lid 6 Select Continue 7 Select Yes in response to Do you want to continue the program The chamber fills with reagent as necessary and the process continues Note The Open User or Open supervisor...

Страница 74: ... on the Standby screen The Select chamber dialog appears A quick clean of recently used chamber s performed by default 3 Press OK to confirm 4 The text box shows the estimated finish time Press Yes to confirm Note After 50 cycles of quick cleaning you must perform an extended cleaning cycle standard cleaning A reminder will appear on the screen 3 6 Cleaning Baskets and Replacing Reagents 3 6 1 Run...

Страница 75: ...empty the used reagent into an appropriate waste container 4 Clean the emptied bottle with a suitable cleaning agent if necessary and then rinse it thoroughly again 5 Refill the bottle with the appropriate reagents according to the levels marked on the side The reagent bottles are marked with two filling lines US gallons on the left and liters on the right If you intend operating with one chamber ...

Страница 76: ...ng and refilling reagents Reagent stations drain and refill through the rotation chamber Before the automated reagent replacement starts you must carry out a clean cycle 1 Place a suitable waste container in front of the instrument Note Automatic replacement Make sure the waste container is large enough for the quantity being drained and is suitable for the reagent 2 Attach the drain hose to the g...

Страница 77: ... end of the hose is in a suitable container then select Yes in response to Is the hose connected to the device and its free end in the external tank 9 The device pumps the reagent into the rotary chamber empties it into the waste container the basket holder rotates for a short time and the device emits an audible signal when the chamber is empty Note You can switch the signal off with the button C...

Страница 78: ... screen 6 Select Yes in response to Fill this station 7 Select Yes in response to Is this station really empty The instrument pumps the new reagent through the rotary chamber into the selected station It spins briefly then sounds an audible signal that filling is complete To prevent the spillage of reagent the tubes are removed after the filling is complete 8 Run a cleaning cycle see Section 3 6 1...

Страница 79: ...he reagent the last filled reagent is stored If the next externally filled reagent is incompatible according to list of the defined Reagent Groups then the reagent above it is used The chamber is filled with the cleaning alcohol rinsed and then the second Reagent stored If more reagents are to be filled including the cleaning alcohol from an external tank first change the cleaning alcohol ...

Страница 80: ...list of its protocol steps and options The area above the list shows the protocol name and the predefined fixed end time if the Fixed end time box is checked 3 To edit a value touch its text box and proceed as follows Reagent Select the desired reagent from the available list and click OK to save the change Pressing OK will save the log or enter additional values Duration or Temperature Select val...

Страница 81: ...p A clock appears Wait steps are protocol dependent and there can only be one wait step per protocol Typically a wait step is defined as a formalin step 4 To insert a step a Touch a number before a step to insert a new step b Touch Insert Step on the right side of the screen c Insert appropriate values as described in step 3 above Alternatively To add a step at the end of the protocol touch an fre...

Страница 82: ...nging protocol steps If a protocol is running you can modify parameters for steps that have not been processed yet For example you can change para meters for step 3 and the following steps while running step 2 However you cannot insert or delete protocol steps and you cannot change the current step Note Protocol changes Changes made to a running protocol are not stored permanently They are only va...

Страница 83: ...hest reagent temperature you can use for a chamber is 65 C Pressure vacuum Among the protocol steps you have the possibility to choose pressure or vacuum or a change between pressure and vacuum You can also define Fixed End Time This defines the duration of a wait step where specimens remain so that the protocol will finish at the programmed end time Note Wait Step There can only be one wait step ...

Страница 84: ... the fixed end time if you want to assign a predefined end time 6 Touch the time box that appears and select the desired end time from the virtual keyboard 7 On the screen touch the white box under reagent select the desired reagent from the list that appears then select OK 3 7 3 Creating a new protocol Routine Operations Editing and Creating Protocols Figure 37 Edit program screen Step 1 20 2 3 4...

Страница 85: ...g the next step 8 Touch the white box under duration select the time you want in minutes from the virtual keyboard then select Enter The minimum time is 1 minute the maximum is 99999 minutes that is unlimited for all practical purposes 9 Touch the white box under Temperature select the desired temperature in Celsius from the virtual keyboard then select Enter A zero here means that this step is no...

Страница 86: ... the protocol to wait in Adding a wait step Add the wait step only in steps in which an extended retention period of the sample will not be impaired These are usually fixed steps 14 You can see all the protocol steps listed in the table Use the buttons on the right to scroll through the steps and insert or delete steps if necessary Routine Operations Editing and Creating Protocols Step 1 20 2 3 4 ...

Страница 87: ...en displays an appropriate error message OK will not be an option if there are issues with the protocol 16 Select the option Open User with the appropriate button that is located next to the log list if you want to open the chamber during an active protocol only at the User Level Note The Open User or Open supervisor applies to all protocols in each chamber Note The device can not detect whether t...

Страница 88: ...floppy disk Make sure the disk is formatted with MS DOS before you use it then insert it into the integrated floppy disk drive and select Export Note Devices with the monitor interfaces for peripherals do not have a floppy drive USB data stick Plug the included USB data stick into one USB port on the left side of the device or the left side of the screen and select Export Protocols are stored on t...

Страница 89: ...USB port or insert the disk Note Devices with the monitor interfaces for peripherals do not have a floppy drive 2 Choose the appropriate chamber by selecting either Rotation or Biopsy 3 Select New and highlight the empty box 4 Select Import 5 Select Yes to overwrite the new empty protocol 6 Select the protocol from the list and select Open Note Invalid file If the file you want to import is corrup...

Страница 90: ...ocessing parameters 1 On the Standby screen press Reagents The Reagents management screen appears 2 On the touch screen select the paraffin container you want by pressing the appropriate container icon The functions available appear in the lower part of the screen 3 Press Regenerate and confirm it with the button Yes The instrument pumps paraffin from the selected container into the rotary chamber...

Страница 91: ...etup to access the setup menu 2 Select Quick Start A list of assigned and vacant buttons appears Note Quick start Button 1 The first quick start button Button 1 has already been assigned as a default cleaning program Do not delete it Cleaning cycles can only be started by selecting the quick start button 3 To create a new button touch a vacant line in the list A selection list appears 4 Press Edit...

Страница 92: ...ically assigned to the button 6 Touch the field to the right of Text in the middle of the screen and enter a program description of not more than 10 characters Confirm with Enter 7 Select OK The quick start button is created and becomes available on the Standby screen Routine Operations Creating and Changing Quick Start Buttons Edit program Cancel OK Type Rotation Biopsy Clearing Text max 10 char ...

Страница 93: ...s These give you information on system readiness for example the temperature of the paraffin reservoir and the progress and status of the protocols currently in use Maintenance messages These provide information about routine maintenance for example station cleaning chamber cleaning reagent expiration and so on Error messages These alert you to various types of system malfunctions for example rota...

Страница 94: ... closed There is a blockage in the air or reagent lines Appropriate error messages indicate the specific station that must be cleaned to remove the blockage for example Cleaning Station X Station X filling time emptying time is too long a station cleaning is recommended Station cleaning takes approximately 20 minutes 1 Touch OK to confirm the error message 2 Select Setup from the screen menu 3 Sel...

Страница 95: ...shed Emptying cleaning program is finished do you want to open the lid Click OK to accept Rotation program Lid is unlocked and can be opened Touch OK to acknowledge this Station cleaning cleaning for station X is deleted Touch OK to acknowledge this Please put the cleaned bottle and the Alcohol cleaning bottle X back in their original positions Touch OK to confirm you have done this The station cl...

Страница 96: ...in step 9 is performed in about 20 minutes The system displays the error message X Please check that the bottles are secured in their positions bottle caps or plugs for leakage and whether level of the substance in the wash bottle is too low 1 Touch OK to acknowledge the error message 2 Check the lids of all the reagent stations and make sure they are properly closed 3 Check that all reagent stati...

Страница 97: ...on of the basket holder and observing it through the glass window of the chamber lid If the basket holder is rotating press Yes The chamber unlocks Open the lid clean the level sensors see Section 5 1 then reclose the lid Press Continue to continue the program If the basket holder is not rotating press No If you can see the cause of the blockage for example an open or misaligned basket or loose sc...

Страница 98: ...intains the rotation for additional 5 minutes When the supply is being emptied back into the tank the protocol in an interrupted manner It is however started a timer to ensure that melting already solidified paraffin has melted completely Paraffin steps in the protocol are executed only when this timer expires If the device was in the paraffin step at the time of the power outage the teo is extend...

Страница 99: ...hamber and Section 4 6 2 Emergency ventilating the rotary chamber before you open the chambers To ventilate the biopsy chamber in the event of a power failure 1 Insert the supplied hexagonal key with a T handle into the lower hole on the left side of the instrument and press so that the vent valve is located there 2 Keep the key pressed for approximately 10 seconds until the ventilation sound is n...

Страница 100: ...f the instrument and press so that the vent valve is located there 2 Keep the key pressed for approximately 10 seconds until the ventilation sound is no longer audible 3 Insert the hexagonal key into the hole below the lid of the rotary chamber from the left side 4 6 2 Emergency ventilating the rotary chamber Troubleshooting and Errors Opening Chambers During Power Failures Figure 43 Opening the b...

Страница 101: ...nd Once the key is inserted into the screw head the screw can be unscrewed A slight resistance and a whirring sound indicate that the key is moving the screw b Turn the key to the left until you can open the lid Note Turn the key counter clockwise only Try to open the chamber after each rotation of the key When the lid opens Do not continue turning the key when the lid has unlocked Figure 45b Plac...

Страница 102: ...ttle is too low Make sure that all reagent bottles are tightly closed and fully inserted in position Check whether the cover of the chamber is closed X Biopsy program The flow resistance in the air line or the water level in the wash bottle is too high Check the level of the wash bottle Reduce the level of the wash bottle to the minimum mark See section 3 3 2 X over under pressure error X A pressu...

Страница 103: ...gs Param 4 Press Log File 5 Use the Up and Down buttons at the top of the log table to choose the day on which the event needs to be analyzed 6 Press Export There are two files DataLog and STPLog Stored on the USB stick 7 Two files STPLog and DataLog are stored on the USB Wait for the Export button to appear 8 Now you can remove the USB stick and plug it into your PC to send the data via email Not...

Страница 104: ...n container and fill if necessary See section 2 4 3 Check for any prompts from the reagent management system and refresh reagents if indicated See Chapter 3 6 Replace the reagent chamber filter in the rotation chamber once or twice a week Loosen the screw which is fixed to the Outlet with a screwdriver or a coin Carefully remove the used filter and replace it with a fresh filter reagents Replace t...

Страница 105: ...here are two cleaning programs a short Short Cleaning and a long Standard Cleaning By default the short program starts the Standard Cleaning must be used after 50 short cleaning programs The device will notify you when 50 short cleaning cycles were performed Note Cleaning liquids Thermo Fisher Scientific recommends that you use pure Xylene and alcohol for cleaning purposes Xylene You must use only...

Страница 106: ...instrument using commercially available cleaning agents and a smooth cloth Use the cleaning agent sparingly so that it does not drip into the instrument or onto the screen Note Cleaning the surfaces For cleaning the outer surfaces of the instrument and its accessories use mild domestic cleaners Do not use aggressive cleaning agents or solvents such as abrasive agents as the paint and plastic parts...

Страница 107: ... local service provider authorized by Thermo Fisher Scientific offers a service contract which guarantees that the routine maintenance takes place on time and that your instrument is always in perfect condition A notification will appear on the screen during these intervals ThermoFisher service should be called as soon as possible The prompt can be closed and the unit used normally until the maint...

Страница 108: ...d as a result of using nonconforming packaging will be fully charged to the sending party Thermo Fisher Scientific reserves the right not to carry out a repair depending on the seriousness of the damage To order original packaging materials contact Thermo Fisher Scientific or a local dealer authorized by Thermo Fisher Scientific Biohazard Make sure you follow the precautions for biological hazards...

Страница 109: ... 9 Dispose of all liquids appropriately according to the safety data sheets and any applicable laboratory regulations 10 Install transport handles as described in Section 2 3 Transporting the STP420 to Its Site 11 Pack the instrument in the original packaging as this gives you the best chance of transporting the instrument without damage Note Original packaging If the original packaging is no long...

Страница 110: ...ectants cleaning liquids and section waste must be disposed of in accordance with the appropriate regulations governing special waste Reagents must be disposed of in accordance with the General Laboratory Guidelines The labeling on the instrument and its literature indicates that after the final shutdown the instrument must not be disposed of with ordinary domestic waste Dispose of your instrument...

Страница 111: ... Xylene cleaning bottle 264550 1 Set Reagent tags 526540 1 Power cord 314990 1 User Guide Gernan 387790 English 387791 French 387792 Spanish 387793 Italian 387893 1 Hexagon key with T grip 362470 1 angled Allen key 362120 1 Funnel 362770 1 Plastic paraffin scraper 334020 1 USB data stick 256 Mbytes 612000 1 Set Hose 1 set of tubes includes two tubes with connections 526860 1 Active coal filter cpl...

Страница 112: ... Start a cleaning cycle Manage expired reagents Change language settings date format in temperature and pressure Turn on and off the acoustic signal at the end of logs and at the end of the automatic filling or emptying operation Access to the log files of error lists Export log files and send troubleshooting e mail to the Thermo Fisher Scientific Service Change password for supervisor B 1 Users A...

Страница 113: ...with users and Run protocols Write Delete Edit Export Import Manage reagent list Create Delete Edit Export Manage reagents Define reagent parameters Assign defined reagents to specific stations General settings Access logfiles and data loggers for troubleshooting Export logfiles and data loggers for Service experts analysis Receive advice and service help from Service experts ...

Страница 114: ...to reset them to the factory defaults automatically Feature Setting Date DD MM YY Language English Paraffin temperature 60 C Protocols Draining time 20 seconds Pressure vacuum OFF Passwords User routine user No password necessary Supervisor Supi Demo STP420 Technician service technician No password necessary however this mode does require a service dongle Appendix C Factory Default Settings ...

Страница 115: ...alins for example Glyoxal based fixative Zink Formalin buffered and non buffered Fast Flex fixative solution 1 Drain Ethanol Isopropanol Fast Flex dehydrant solution 2 Flex dehydrant Wax Paraffin Paraplast Fast Flex paraffin Paraffins with melting points between 55 C and 58 C Clarify Xylene Clear Rite3 Fast Flex Xylene solution 3 Fast Flex Xylene substitute solution 3 Clear Xylene Ethanol Thermo S...

Страница 116: ...s 50 C up to 65 C Paraffin containers Paraffin temperature 55 C up to 65 C Volume per container 4 5 liters Transportation and storage conditions Storage temperature range 0 C up to 50 C Operating conditions Temperature 15 C to 35 C at max relative humidity of 60 Altitude up to 1 500m NN Floor loading requirements 350kg m Power requirements 100 115V 10A 50 60Hz 10 220 240V 5A 50 60Hz 10 Internal pr...

Страница 117: ...6 3 F10 6 3 A Paraffin container heater 4 O6 4 F11 6 3 A Valve unit V5 V6 F12 6 3 A Reserve F13 1 AT Reserve F14 1 AT Magnetic valve biopsy chamber B24 F14 1 AT Magnetic valve pressure P31 F15 1 AT Magnetic valve rotary chamber biopsy chamber P32 F16 1 AT Magnetic valve vacuum P30 F17 1 AT Locking magnet biopsy chamber B24 F18 1 AT Reserve 1 F19 1 AT Reserve 2 F20 1 AT Pollution degree 2 Overvolta...

Страница 118: ... 30 P V 0 20 4 4 80 alcohol 30 P V 0 20 4 5 95 alcohol 60 P V 0 20 4 6 100 alcohol 60 P V 0 20 4 7 100 alcohol 60 P V 0 40 4 8 Xylene 1 30 P V 0 20 4 9 Xylene 2 60 P V 0 20 4 10 Xylene 3 60 P V 0 40 4 11 Paraffin 1 30 P V 62 20 4 12 Paraffin 2 30 P V 62 20 4 13 Paraffin 3 30 P V 62 20 4 14 Paraffin 4 60 P V 62 40 4 Total time including time to fill and empty hr min 11 32 Biopsy 1 Formalin 30 P V 0...

Страница 119: ...lc 100 65 P V 0 60 4 8 Xylene 30 P V 0 20 4 9 Xylene 45 P V 0 60 4 10 Paraffin 30 P V 62 20 4 11 Paraffin 30 P V 62 20 4 Total time including time to fill and empty hr min 08 19 Reagent Time Minutes Xylene cleaner 10 Xylene cleaner 10 Alcohol cleaner 5 Cleaning cycle Short Cleaning both chambers Reagent Time Minutes Xylene cleaner 10 Xylene cleaner 10 Xylene cleaner 10 Alcohol cleaner 5 Cleaning c...

Страница 120: ...s the following practise is recommended 1 Use Turboflow II cassettes with 7 7cm gauze and 2 3cm pieces of filter material that is the maximum size of the cassette 2 Place the histological material on the filter piece arrange and it as necessary 3 Cover the material with a second filter piece 4 Wrap the double layer in the gauze 5 Place the wrapped material in the cassette and close the cassette fi...

Страница 121: ...0 99 100 105 button command 24 70 83 quick start 27 74 91 C cassettes 22 27 58 71 108 120 chamber biopsy 16 30 99 100 105 level sensors 97 opening 31 99 process 18 22 rotary 18 36 69 77 90 charcoal filter 18 22 66 cleaning cycle 25 50 74 78 90 105 119 liquids 105 110 reagent 10 58 105 119 station 18 35 94 command buttons 24 70 83 conventions 11 D drain hose 64 65 76 rail 62 106 time 53 71 81 86 90...

Страница 122: ...ntilation 99 F filter charcoal 33 66 reagent 34 104 I installation 9 39 integrated hard disk 18 K keypad alpha numeric 18 29 55 numeric 28 L language 56 level sensors 97 104 M maintenance 6 9 34 64 95 104 message error 12 22 77 78 87 maintenance 93 progress 18 status 93 MSDS 35 N numeric keypad 28 ...

Страница 123: ...n 70 80 exporting 88 importing 88 new 26 84 105 predefined 26 36 70 80 progress 26 72 93 start time 56 70 steps and options 80 83 timing 36 Q quick start buttons 22 27 74 91 92 R reagent 11 44 54 75 assigning 35 59 67 cleaning 10 58 105 119 color 55 77 consumption 78 editing 26 51 54 57 filter 104 flammable 58 59 71 groups 51 54 58 new 59 replacing 74 76 stations 18 35 67 unavailable 70 reagent fi...

Страница 124: ... settings 50 reagent management 57 67 reagent setup 50 setup 57 standby 23 self test 22 72 102 station cleaning 18 33 86 paraffin 69 reagent 18 35 94 status window 23 48 symbols 8 11 T temperature 18 27 47 50 53 69 71 touch screen 11 18 23 28 transportation 108 transport handles 41 43 U USB data stick 20 54 60 88 user access 66 90 level 31 using two chambers 32 46 75 V ventilation 10 22 99 100 ...

Страница 125: ...125 Thermo Fisher Scientific User Guide STP420D STP420ES Index W wait step 70 81 86 wash bottle 18 22 45 48 64 waste disposal 11 110 water level 48 64 96 102 ...

Страница 126: ...ermo Fisher Scientific User Guide STP420D STP420ES Anatomical Pathology Otto Hahn Strasse 1A 69190 Walldorf GERMANY 49 6227 836 200 4481 Campus Drive Kalamazoo MI 49008 USA 1 800 522 7270 www thermofisher com ...

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