Thermo Scientific Dionex ICS-1100, Руководство оператора

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Dionex ICS-1100 Ion Chromatography System
84
Doc. 065289-03 10/12
5.1.2 Calibrating the Conductivity Cell
When to Calibrate
•
After every 6 months of use
NOTE Do not use this procedure to calibrate a new cell. If you
are replacing a cell, see the instructions in Section 5.10
to install the new cell and calibrate it.
1. Open the Dionex ICS-1100 Wellness Panel in Chromeleon (see
Section 5.1.1 ).
2. On the Wellness Panel under Electric Conductivity Cell
Calibration , click Offset Cal .
3. When the offset calibration is complete, click Slope Cal on the
Wellness Panel.
4. Disconnect the pump output line from the injection valve.
5. Disconnect the line from the suppressor
ELUENT OUT
port to the cell
inlet and connect the pump output line directly to the cell inlet.
6. Disconnect the line from the suppressor
REGEN IN
port to the cell
outlet. Direct the cell outlet line to a waste container.
7. Verify that the backpressure is at least 7 MPa (1000 psi).
8. On the Wellness Panel under Conductivity Cell Calibration , click
Cell 35 °C . Allow the cell to reach this temperature, and then wait an
additional 5 minutes to let it stabilize.
9. Select 1.00 mL/min to begin pumping 1.00 mM KCl through the cell
at 1.0 mL/min.
10. Wait until the total conductivity reading stabilizes (in about
15 minutes) and then select
Calibrate
on the Wellness Panel.
Items Needed Description
1.0 mM KCl solution Prepare by dissolving 0.07456 g of reagent-
grade KCl in 1 liter of 18-megohm DI
water.
Backpressure tubing to provide
at least 7 MPa (1000 psi) Use 0.076-mm (0.003-in) ID yellow PEEK
tubing (P/N 049715).