Thermo Scientific Dionex Easion, Руководство оператора

Страница: 42 / 126

Dionex Easion Operator’s Manual
34
Doc. 155028-01 08/20
3.7 Equilibrating the System
1. Click the pump On button on the Chromeleon ePanel.
2. Select the flow rate recommended for the column and allow the system to
equilibrate. The Chromeleon ePanel displays the background conductivity
(the conductivity of the eluent without the offset performed by the autozero
command).
3. Click Autozero on the Chromeleon ePanel to offset the background and zero
the conductivity reading.
4. Monitor the system pressure to make sure it is at the expected pressure for the
installed column (refer to the column manual for details) and is stable.
Note that column manuals typically provide pressure specifications for
systems that
do not include a guard column. If a guard column is installed in
the Dionex Easion, the system pressure will be 15% to 20% higher than the
specification in the column manual.
•
If the pressure is less than expected, air may be trapped in the system. To
release the air, temporarily remove the pump fitting (
P
) on the injection
valve. After allowing the air to escape, reconnect the fitting.
•
If the pressure is too high, there may be a restriction in the system
plumbing. For troubleshooting guidance, see
Section 4.7 .
5. Check for leaks in the regenerant bottle.
6. Check that liquid is flowing out of the suppressor
REGEN OUT
waste line.
7. Monitor the baseline conductivity. In general, this should be <30

S/cm for a
system set up for anion analyses or <2

S/cm for a system set up for cation
analyses. Equilibration time varies, and it can take some time to reach the
expected value.
•
If the conductivity is too high, see Section 4.12 for troubleshooting
guidance.
•
If the baseline is drifting or there is excessive “noise” (large fluctuations
in readings), see
Section 4.13 .