Terumo BCT Quantum Cell Expansion System, Руководство оператора

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Rapid IC Washout
The purpose of this task is to remove non
‑
adherent cells from the bioreactor. This task imposes
ultrafiltration onto the fiber across the entire fiber length.
Table 9-3 describes the types of solution that are needed to attach to each line when performing Rapid
IC Washout. These solutions and corresponding volumes are based on the factory default settings for
this task.
Table 9-3: Solutions for Rapid IC Washout
Line
Solution Attached to the
Line
Required Volume (estimate based on factory
default settings)
Cell None N/A
Reagent None N/A
IC Media Media with protein 0.6 L
Wash None N/A
EC Media Media without protein 0.1 L
Rapid IC Washout pathway:
Task
>
Washout
>
Rapid IC Washout
1. Confirm the settings for Rapid IC Washout shown in Table 9-4.
Table 9-4: Settings for Rapid IC Washout
Setting Factory Default Setting Options
IC Inlet IC Media Reagent, IC Media, Wash, EC Media
IC Inlet Rate 260 mL/min 50 to 500 mL/min
IC Circulation Rate −45 mL/min N/A
The system automatically calculates this rate based on the IC Inlet Rate
for this task.
EC Inlet EC Media Reagent, IC Media, Wash, EC Media
EC Inlet Rate 40 mL/min 0 to 100 mL/min
EC Circulation Rate 30 mL/min 10 to 300 mL/min
Outlet IC Outlet N/A
Rocker In Motion (−90°, 180°, 1 sec) In Motion (−180° to 270°, 0 to 3600 sec), Stationary
(−180° to 270°)
Rapid IC Washout
Quantum
®
Cell Expansion System • Operator's Manual for Software Version 2.1 9-3