Terumo BCT Quantum Cell Expansion System, Руководство оператора

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Add Bag Contents
The purpose of this task is to load a fluid, such as a reagent, into the IC circulation loop until the bag that
contains the fluid is empty. The IC outlet valve is closed during this task. This task includes two separate
steps:
•
Step 1: loads a fluid into the system.
• Step 2: chases the fluid from the ARC into the IC circulation loop.
Table 11-7 describes the types of solution that are needed to attach to each line when performing Add
Bag Contents. These solutions and corresponding volumes are based on the factory default settings for
this task.
As a precondition before starting this task:
Fill the cell inlet bag with a minimum of approximately 40 mL of air.
Table 11-7: Solutions for Add Bag Contents
Line
Solution
Attached to the
Line
Required Volume (estimate based on factory default settings)
Cell None N/A
Reagent Reagent As desired
IC Media Media with protein 0.1 L
Wash None N/A
EC Media None N/A
Add Bag Contents pathway:
Task
>
Feed and Add
>
Add Bag Contents
1. Confirm the settings for step 1 shown in Table 11-8.
Table 11-8: Step 1 Settings for Add Bag Contents
Setting Factory Default Setting Options
IC Inlet Reagent Cell, Reagent
IC Inlet Rate 10 mL/min 0 to 100 mL/min
IC Circulation Rate 100 mL/min −300 to 300 mL/min
The system automatically calculates this rate based on the IC Inlet Rate
for this step.
EC Inlet None N/A
EC Inlet Rate 0 mL/min N/A
Feed and Add Tasks
11-8 Quantum
®
Cell Expansion System • Operator's Manual for Software Version 2.1