ACCQPrep HP125
Section 6 Maintenance
6-24
Figure 6-34 Check valve holder
2. Remove check valves from pump head and place in a bea-
ker of methanol. Sonicate check valves for 15 to 20 min-
utes.
3. Reinstall check valves into pump head making sure that
the ends of the check valves (with multiple holes) are fac-
ing up towards the outlet of the pump.
4. Reinstall check valve holders and tighten finger tight.
Then using pliers tighten an additional ¼ turn.
5. Check the flow rate delivery. If the flow rate delivery is still
incorrect, replace the check valves. The correct flow rate
depends on the back pressure of the carrier solvent. The
flow will be 0.5 mL/minute until the fluid interface reaches
operating pressure, then reduces the flow rate to 0.2
mL/minute under typical operating conditions for the fluid
interface. The operating mode can be determined by listen-
ing to the pump operate. While running at 50 mL/min, the
pump motor speed is continuous. Once operating pressure
is reached, the pump has a rapid refill stroke approxi-
mately that occurs ~ 8 seconds.
6.8.7 Replacing Check Valves
1. Install new check valve cartridges (P/N: 250-0001-17) into
pump head housing making sure the ends of the cartridges,
with three small holes, are facing upwards towards the
outlet (Figure 6-35).
Figure 6-35 Correct orientation of cartridge
2. Tighten check valve holders finger tight, then with pliers
tighten an additional ¼ turn.
6.8.8 PurIon Cone Cleaning
This error occurs if the capillary inlet to the PurIon vacuum
region is restricted causing a higher vacuum reading than
normal. The PurIon S & L models compatible with the NextGen
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