Teledyne ACCQPrep HP125, Инструкция по установке и эксплуатации

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ACCQPrep HP125
Section 4 Operation
4-2
4.3 Starting a Separation
To separate a single sample, press P
LAY
to start a separation.
The M
INIMUM
R
UN
R
EQUIREMENTS
screen is displayed. This
allows you to select an injection technique and select where the
fractions will be collected (the starting rack and tube position). In
addition, the system projects the amount of solvent required and
the separation duration. To proceed from this screen, the S
TART
E
QUILIBRATION
button is selected.
For an ELSD If an ELSD is installed on the system, this screen has the option
of using the ELSD or disabling the ELSD. ELSD method param-
eters are typically based on the solvents used and therefore are
somewhat universal when doing preparative separations of non-
volatile samples. Semi-volatiles may require optimization of the
ELSD parameters. Peak delay compensation is based on using
water and methanol or water and acetonitrile and is selected
based on the names assigned to the solvents.
Note
The M
ETHOD
E
DITOR
should be used to change the ELSD
parameters.
For a PurIon If a PurIon mass spectrometer is connected, the R
UN
R
EQUIRE
-
MENTS
screen allows some customization of the PurIon operating
conditions. This allows the use of default or commonly used
methods while allowing customization of the separation for both
masses used for fractionation or mass loading of sample sent to
the PurIon for detection. (Low mass load is the default and sends
the minimum amount of sample to the PurIon for detection. Due
to the high concentrations of sample in a preparative separation
this is adequate for most samples with good ionization.) Further
information can be found in Section 5.2.11.
If No AutoInjector or
AutoSampler are
Connected If you do not have an AutoInjector or AutoSampler, the
ACCQ
Prep will give you the option of injecting the sample imme-
diately or after column equilibration.
• L
OAD
N
OW
- If your sample is ready you can inject it into
the syringe port on the side. Press S
TART
E
QUILI
-
BRATION
, and walk away.
• L
OAD
A
FTER
E
QUILIBRATION
- Select this if you want to
give your column time to equilibrate while you finish
preparing your sample. Once the equilibration is
complete, the system will pause and prompt you to inject
your sample.
The next section will cover how to start a separation when the
additional modules are set up.
4.3.1 Separation with an
AutoInjector
To use the AutoInjector Module:
1. Place the sample probe into your sample container.
2. Press P
LAY
to start a separation as you would for an indi-
vidual separation.