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Version 1 .0

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Specifications

Dimensions

Electrical

Width

120 cm (47 in)

Model Number

EasyPREP

BOD-300

Depth (Total)

81.3 cm (32 in)

Voltage

115~230V

Depth (Base)

63.5 cm (25 in)

Power

130W

Height

64 cm (25 ¼ in)

Frequency

50/60Hz

Environmental

Relative Humidity

30% to 80%

Altitude

up to 2000 m

Installation

Category ll

Pollution

Degree 2

Ambient Operating Temperature

15 °C to 40 °C

ProODO Probe Specifications

The specifications listed below are system specifications for the ProOBOD probe when used with a BOD-300 instrument.
These specifications are subject to change without notice.

Dissolved Oxygen (mg/l)

Temperature (°C)

Sensor type

Optical (dynamic luminescence quenching lifetime detection)

Range

5 to 70 °C

Range

0 to 50 mg/L

Accuracy

±0.2 °C

Accuracy

0 to 20 mg/L ±0.1 mg/L or 1% of reading, whichever is greater 20 to 50 mg/L
±10% of reading

Resolution

0.1 °C

Resolution

0.01 or 0.1 mg/L (auto-scaling)

Barometer

(mmHg)

Range

375 to 825 mmHg

Accuracy

±1.5 mmHg from 0 to 50 °C

Resolution

0.1 mmHg

Fuses-Ratings

The Power Supply has an over voltage, overload and short circuit protections.

NOTE

Содержание EasyPREP BOD 300

Страница 1: ...User Manual www scpscience com EasyPREP BOD 300 ...

Страница 2: ...Bod probe 22 6 5 Admin Tools Tab 23 6 5 1 Robot 23 6 5 2 Rack Parameters 24 6 5 3 Calibration of the Autofill Sensor s 26 6 5 4 Instrument Memory Parameters 27 6 5 5 QC Controls 27 6 5 6 Parameters 28 7 Guidelines on BOD CBOD Analysis 29 7 1 Priming 29 7 2 Peristaltic pump calibration 29 7 3 Probe Calibration 29 7 4 Typical BOD testing Procedure 29 7 4 1 Sample Pretreatment 29 7 4 2 Dilution Water...

Страница 3: ...ris France Insurance covering the full value of the shipment is included with the transportation charges If you wish to select a specific carrier and or have insurance to cover your shipment please contact us immediately Claims SCP SCIENCE takes great care in the packaging of goods prior to shipment In the event that breakage occurs during shipment claims for damaged merchandise must be made to th...

Страница 4: ...odates 60 ml 165 ml or 300 ml bottles for optimized throughput allowing the analysis of up to 60 position 300 ml bottles 72 position 60 ml bottles or 72 position 165 ml bottles without operator intervention Biochemical Oxygen Demand BOD analysis is an empirical bioassay type procedure which determines the dissolved oxygen consumed by microbial life while assimilating and oxidizing the organic matt...

Страница 5: ... is used to emphasize information pertaining to procedures that if not strictly followed may result in damage or destruction to the instrument or improper instrument operation WARNING A warning is used to emphasize information about dangerous or hazardous conditions relating to the operation cleaning or maintenance of the instrument that may result in personal injury NOTE A note is used to emphasi...

Страница 6: ...Products are supplied for laboratory use only and should not be used for any household medical or therapeutic application SCP SCIENCE presumes that only trained and qualified individuals familiar with procedures suitable for the safe operation of these instruments will handle them Our customers are solely responsible for the safe operation handling and use of these products Minimum safety consider...

Страница 7: ...and fingers free of the system when it is moving to reduce the risk of pinching Always plug the EasyPREP BOD 300 in a properly grounded three prong electrical outlet 100 240V receptacle Use a stabilized constant voltage AC power supply with a voltage within 5 of the specified level Always use the provided mains power cord or one with the same manufacturer s specifications If the BOD 300 is used in...

Страница 8: ... system specifications for the ProOBOD probe when used with a BOD 300 instrument These specifications are subject to change without notice Dissolved Oxygen mg L Temperature C Sensor type Optical dynamic luminescence quenching lifetime detection Range 5 to 70 C Range 0 to 50 mg L Accuracy 0 2 C Accuracy 0 to 20 mg L 0 1 mg L or 1 of reading whichever is greater 20 to 50 mg L 10 of reading Resolutio...

Страница 9: ...e shown 5 Probe Holder 6 Table 7 Rinse Pump 8 10 pin connector 9 Dilution Pump 10 Seed Pump Label Description 11 Inhibitor Pump 12 Dilution Pump Optional 13 Inhibitor Pump Optional 14 Seed Pump Optional 15 Power in receptacle 16 Power Indicator 17 Power ON OFF Button 18 USB Receptacle 19 Autofill sensor 20 Decapper 10 12 13 14 9 11 Figure 1 Rear View 5 7 8 19 20 ...

Страница 10: ... has a minimum clearance of 8 in 20 cm 5 2 1 Installing The BOD Racks Place the BOD racks on the top table using the positioning pins provided on the top table to align them Bottle caps must be removed by hand prior to starting sequence unless the BOD 300 decapping capping accessory is installed The BOD 300 decapping capping acccesory is not compatible with acrylic caps BOD 65 ml 300 ml PE bottle ...

Страница 11: ...1 0 11 5 3 before Software Installation 5 3 1 Computer System Requirements PC Processor 1GHz or superior RAM memory 1 GB RAM Disk min space 1 GB free Ports 1 USB port available Operating System Windows 7 and 10 ...

Страница 12: ...s tab Where results are displayed in real time Utilities tab Where the basic method parameters can be adjusted in addition to 1 Probe calibration and reading 2 Liquid dispensing parameters 3 Priming Cleaning Admin Tools tab Password protected used to configure the system in the following ways 1 Motion speed when manually setting the positioning of robotic arm 2 Liquid dispensing parameters speed d...

Страница 13: ...ng the tests 6 1 3 Hardware Detection Figure 6 Indicators to show the status of communication with hardware The six 6 for dual probe configuration and 4 for single probe configuration indicators at the bottom of the screen indicate whether the modules have been detected by the instrument A red indicator signifies that a module has not been detected or is faulty A green indicator signifies that the...

Страница 14: ...sented by a color in the rack visualization Figure 9 Legend field Once the Run button is pressed the indicator representing the bottle in process turns blue Once the analysis is complete the indicator representing the bottle in question turns green If the measurement of the bottle is not within an acceptable for Day 0 reading the bottle in in question turns orange The system will prompt the user t...

Страница 15: ...n is stored in the results file This is only stored after the run button has been pressed Comment Remark A field for any comments This information is stored in the results file This information is only stored after the run button has been pressed Figure 11 Operator s Name and Comment Rack fields 6 2 2 Process Sample Field Figure 12 Input sample field ...

Страница 16: ...n samples If rinse is checked the probe is washed between each bottle Unchecked between bottles and checked between samples to rinse probes between samples Sample ID Unique ID of the sample If the sample ID is the same as another sample the software will calculate the average value based upon it meeting the QC requirements VOL ml Volume in ml of the sample pipetted into the bottle DL Dilution fact...

Страница 17: ...will list all results from probe 1 followed by probe 2 Sorting by Bottle will list the samples in sequential order 6 3 1 Definitions All the following parameters are stored in the results file Date A field for the date of test Operator A field for the operator s name Cell label and cell value Inspired by an Excel table in order to show the clicked cell on the results table and its value Rack Posit...

Страница 18: ...Oxygen measured in the final test Final Temp C Temperature of the sample during the final test Depletion Calculated by subtraction of final DO from initial DO BOD cBOD mg L O2 Calculated sample DO depletion Remark Comments on data out of range In Remarks a bottle which failed measuring criteria will be marked with R If remeasured and bottle passed the criteria RM will appear to the right of R 6 4 ...

Страница 19: ... user definable Select Bottle Choose capacity of bottles to be used Total Volume Actual volume of the BOD bottle Figure 17 Configuration Process field 6 4 2 Position Robot POSITION ROBOT Manually moves the robot arm to the designated rack and bottle When decap is installed the robot will remove caps Rack Choose the rack position Bottle Choose the bottle position Use Probe Move the arm to lower the...

Страница 20: ...mp generally 10 20 ml Press Run Pump and collect the dispensed water in a pre weighed bottle Measure the dispensed water convert to volume and type the value in Measure Press Calibrate the new pump flow rate will be reset under Increment Only press Calibrate ONCE 6 4 3 2 Dilution water pump Calibration function of the pump for dilution water addition Figure 19 Move the robot arm to one of the bott...

Страница 21: ...lysis or clean the tubing fill the tubing with a cleaning solution Inhibitor Seed Pump Set volume to prime clean the inhibitor pump SEED PUMP Set volume to prime clean the Seed Pump Dilution Water Pump Set volume to prime clean the dilution water tubing Figure 20 Priming and Cleaning 6 4 5 ProODO Probe Barometric pressure Reads the barometric pressure Temperature Reads the sample temperature Disso...

Страница 22: ...bottle After reaching 100 water saturation in the air of the bottle about 10 minutes new calibration information is obtained Fill a BOD bottle with 40 ml clean water and place it in position A1 Press Calibrate When prompted for a calibration time type 10 and then press Enter New calibration information is obtained and saved Though drift is minimal over time to ensure the best precision it is recom...

Страница 23: ...position Init Z Individually initializes the Z axis The robotic arm moves the Z axis up to home position Initialization Initializes each axis sequentially starting with Z followed by Y and then X Axis X Allow for direct displacement along the X axis based upon the value given in mm Axis Y Allow for direct displacement along the Y axis based upon the value given in mm Axis Z Allow for direct displa...

Страница 24: ...X Y and Z parameters The racks are configured using bottle 1 back left corner of each rack Figure 23 The racks are configured using bottle 1 back left corner of each rack Figure 28 Figure 23 Rack set parameters X0 Y0 Coordinates of the first bottle within the rack Modify Redefines the X0 and Y0 of a highlighted rack using the values placed in X0 and Y0 respectively Choose a rack Allows for differe...

Страница 25: ...fication dXT dYT Distance between the first and the last bottle within the rack along each axis Increase or decrease values of if the probe s are not place properly Zd Depth of probe when going into the bottle Zp Depth of probe when going into the wash station Allows access to extended settings decapping height autofill sensor height etc Figure 25 Z Auto Fill Depth of Auto Fill sensor when filling...

Страница 26: ...ne 5 in the EasyPREP Memory Parameters as shown in Figure 26 Alteration of the highlighted value will result in a shift in the x axis of the Autofill sensor and the pumps Increasing this value will result a shift to the left and decreasing the value will result in a shift to the right Figure 26 The Z coordinate of the Auto Fill Sensor is changed in ZAP as seen in Figure 25 Z auto fill represents t...

Страница 27: ...es of Type Seed Control minimum allowed oxygen depletion in the seed controls Smaller seed control depletion values will not be used to calculate the seed contribution factor Default values EPA 2 mg L O2 ISO 2 mg L Sample depletion for bottles of Type Sample minimum allowed oxygen depletion in the sample Smaller sample depletion values will be flagged as invalid Default values EPA 2 mg L O2 ISO fo...

Страница 28: ...is not within the remeasure range as defined in Utilities the bottle in in question turns orange The system will prompt the user that bottles have not passed criteria and will give the user the opportunity to remeasure the bottles in question The probes will wash between each bottle In Remarks a bottle which failed measuring criteria will be marked with R If remeasured and bottle passed the criter...

Страница 29: ...tion of the software in Utilities to fill the tubing lines with water 7 3 Probe Calibration Perform a calibration of the probe following described procedure section 6 4 5 1 7 4 Typical BOD testing Procedure Follow established protocols for the preparation of dilution water seed nitrification inhibitor and sample dilutions Nitrification inhibitor should be added either to the seed water or directly...

Страница 30: ...ered but decanted if needed If sparging of the seed water is needed use clean tubing dedicated only for this purpose If running a cBOD batch add your nitrification inhibitor to your seed water or add the inhibitor manually to your BOD bottles 7 5 QC sample GGA standard Prepare Glucose Glutamic Acid GGA standard as described in established methods Note on preparation Pipette in each BOD bottle used...

Страница 31: ...ecessary modifications on the populated sequence Click on Run button to run the sequence Dilution water and seed nitrification inhibitor are added to the bottles containing samples manually poured in After liquid dispensing the probe lowers into the BOD bottle and measures the dissolved oxygen The probe is rinsed in the rinse station between each sample if rinse is chosen The Day 0 measurement is ...

Страница 32: ...columns labelled Depletion and BOD CBOD5 7 respectively Once the analysis is completed the result table is stored in the folder path C Program Files x86 BOD300 Results 7 7 3 Process troubleshooting Do not pair seed blank with the QC sample when using the dual probe BOD 300 such as in Figure 32 The seed efficiency and blank correction must be completed prior to the determination of results Figure 3...

Страница 33: ...w cable probe assembly already has a sensor cap installed and the sensor cap coefficients are preloaded into the probe during production Preparing the probe for the first time A new ProOBOD probe will have a clear protective cover placed over the sensor cap to ensure the cap remains moist during shipment Carefully remove this clear cover from the end of the probe by pulling it straight off the sen...

Страница 34: ...r If the probe is powered on more than this the sensor cap will need to be replaced more often To extend the sensor cap s usable life turn the instrument off when not in use This could mean turning the instrument off over night but not in between readings The sensor cap will also need to be replaced if it is damaged or cracked When replacing a Sensor Cap the Sensor Cap Coefficients must be manuall...

Страница 35: ...or probe with a lens tissue Clean the clear surface of the optical DO sensor window with a lens cleaning tissue Remove the new sensor cap from its hydrated container and dry the inside cavity of the sensor cap with lens tissue Make sure that the cavity is completely dry and clean before proceeding with the installation Using a clockwise motion thread the new sensor cap onto the probe assembly unti...

Страница 36: ... 2 Sensor Storage 8 2 2 1 Sensor short term storage When the Pro0BOD is not in use it MUST BE STORED IN A MOIST ENVIRONMENT i e the sensor either immersed in water or in water saturated air If the sensor cap is allowed to dry out by exposure to dry air it is likely to drift slightly at the beginning of its next use unless it is rehydrated If this occurs follow the rehydrating instructions in this ...

Страница 37: ...lling pump open the software and verify if the pump is working properly Figure 36 Peristaltic Pump After servicing peristaltic pump s flow rate calibration need to be performed in software for proper accuracy 9 2 New Dilution Pump Calibration In Admin Tools Parameters set the Dilution Water Pump Delay to 0 s In Utilities set the Dilution Water Pump Flow Rate to 14 5 ml s Put the end of the dilutio...

Страница 38: ...tab The slope 12 36 of the right curve is the flow rate Type this flow rate in the field Dilution Water Pump flow rate in the Utilities tab Validate these settings by repeating step 3 7 If the off from expected does not meet the requirements start again from step 10 Once the off requirements are met the new dilution pump calibration is finished 9 3 New Seed Pump Calibration Put the end of the seed...

Страница 39: ... pump liquid Check pump tubing and fittings Replace peristaltic pump tubing Invalid DO Value at day 0 Verify that the proODO probe was properly maintained and not damaged Assuming that the peristaltic pump calibration the priming and DO probe calibration were done properly verify that a well diluted sample pretreatment with a pH 7 0 to 7 2 at 200 C was used Verify that the dilution water and the s...

Страница 40: ...France Germany UK Phone 1 800 361 6820 Fax 1 800 253 5549 Phone 1 800 361 6820 Fax 1 800 253 5549 Phone 33 0 1 69 18 71 17 Fax 33 0 1 60 92 05 67 Phone 49 0 8342 89560 61 Fax 49 0 8342 89560 69 Phone 0 800 368 6147 Phone 44 0 7561 7028 90 Corporate Phone 1 514 457 0701 Fax 1 514 457 4499 www scpscience com sales scpscience com ...

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