Qiagen QIAsymphony AS, Руководство по эксплуатации

Страница: 78 / 216

QIAsymphony SP/AS Consolidated Operating Guide 12/2017 78
Important
If you are using sample tubes that are not labeled with bar codes and that are in
different inserts, use either one insert type per tube carrier or leave at least one
position empty between different types of insert.
Important
If you are using sample tubes that are not labeled with bar codes and the
QIAsymphony SP has a configuration other than configuration 3, tubes
containing smaller volumes of liquid or clear liquids may not be detected. In this
case, use a blank bar code label to enable detection of the sample tube.
Continuous loading
It is also possible to load and queue additional samples when a run is already in progress. In this
mode, you can only assign Assay Control Sets that are compatible with the currently loaded
reagent cartridge.
Continuous loading on the QIAsymphony SP is possible for up to 96 samples in any number of
batches, provided that the consumables drawer is fully loaded before commencing the first batch.
After loading the samples, the system allows the user to correct bar code reading errors, change
labware, assign Assay Control Sets, and define the elution slot and volume.
Before starting a run with continuous loading, ensure that:

Additional reagents that are required (e.g., ethanol, buffer in 60 ml buffer bottle) are loaded.

The elution rack is correctly positioned in the “Eluate” drawer before setting up a batch. If you
need to assign an elution slot during batch setup (while a run is active), only the slots that
already contain racks may be used.

Elution racks are set up before a run is started. This ensures that multiple scans of the “Eluate”
drawer are not performed. Every time a scan is performed, the current run is paused.
Unloading a tube carrier
If the tube carrier slot is not locked (LED is not illuminated red), the tube carrier can simply be
removed from the slot. The tube carrier can be removed as soon as the samples have been
transferred.
Depending on the batch status, different actions can be carried out after removing the tube
carrier.