PerkinElmer Raman Flex 400 Series, Руководство по началу работы

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Using the RamanFlex 400 Series . 53
NOTE: If Spectrum software stops responding, refer to Troubleshooting
starting on page 71
for details of how to shut down the instrument and restart the software.
SOP-3: Setting-Up Experimental Parameters and
Collecting Data
This SOP is designed to act as a quick guide to help you start collecting data. The exact
setup for optimized spectral collection is sample dependent, and we recommend that you
modify these settings to suit your needs.
NOTE: If you are using a Triggered Fiber Optic Probe, refer to the
Raman Triggered Fiber
Optic Probe
leaflet (L1321940) supplied with the probe for configuration and usage
details.
1. Ensure the RamanFlex 400 is switched on.
If not, follow SOP-1.
2. If you did not connect to your instrument on starting Spectrum software, select
Instruments
from the Setup menu and then
Select Instrument
from the sub-menu.
The Instrument Connection dialog is displayed.
3. Select your Raman instrument and then click
Connect
.
4. If required, select the probe you want to use as the
Accessory
on the Setup
Instrument Basic tab.
See
SOP-4: Selecting the Fiber Optic Probe
for further information.
5. Using the Instrument Settings toolbar, check and set the instrument parameters, such
as the Start and End points of the scan range (in Raman Shift, for example, 3600 cm
−1
to 100 cm
−1
), and enter a unique Sample ID and Description.
6. Set the
Exposure Time
and number of
Accumulations
.
You can define the Accumulations as a number of Exposures, or as a number of minutes
or seconds.
Typical values for Exposure Time and Accumulations are 5 seconds and 5 exposures,
respectively. Spectra may be optimized by increasing or decreasing the Exposure Time
and number of exposures. To obtain a good signal-to-noise ratio, you should co-add a
number of spectra. This can be carried out by choosing a suitable number of exposures.
7. If required, adjust the
Data Interval
in Raman Shift (cm
−1
).
The default data interval is 2 cm
−1
. The Data Interval can be set on the Setup
Instrument Basic tab. To display the Setup Instrument tabs, select Raman
Instrument from the Setup menu. The tabs are displayed in the dialog pane.
8. If you wish to edit the data collection parameters, such as how background scans are
collected, select the Setup Instrument Data Collection tab.
9. In the Background Exposures section, we recommend that you select As samples.
This sets the number of background scans to the same value as the number of sample
scans.
You may also choose one of the Background Collection options to define when a
background will be taken.
10. If required, select any Auto Processing options.
These options if selected will be applied to all spectra collected, and cannot be undone.