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cBot 2 System Guide
73
Troubleshoot Flow Check Failure
Perform the following procedure to troubleshoot flow check failure. Do not select the option
to bypass flow check until you have completed this procedure to determine the following
conditions:
}
The flow cell is properly positioned on the instrument.
}
The manifold and hardware are working properly.
NOTE
Bypassing the flow check can result in unsuccessful clustering of some lanes.
Because different flow cell types use different flow checks, make sure that you use the
correct recipe, manifold, and flow cell combination.
1
Make sure that you have enough HT1 in row 1 of the reagent plate, and replenish as
needed.
2
Note which lanes failed the flow check. This information is provided on the top-left
corner of the interface screen.
}
If all 8 lanes failed, the flow cell is probably loaded improperly. Remove the
manifold and confirm that the flow cell holes are facing upward, and the flow cell
orientation is correct.
}
If only some lanes failed, the flow cell might not be seated. Remove the manifold,
reseat the flow cell, and then reinstall the manifold.
3
Select
Rerun Check
to repeat the flow check a second time.
4
If the flow check fails a second time, note which lanes failed the flow check, and do 1
of the following:
}
If all 8 lanes failed, you probably have a faulty manifold. Replace it with a new
manifold.
}
If different lanes failed, you probably do not have a faulty manifold. Inspect the
volumes of HT1 in row 1 to make sure that the tubes contain equal volumes.
5
Select
Rerun Check
to repeat the flow check for a third time.
}
If this flow check fails after replacing the manifold, go to step
}
If this check fails and you did not need to replace the manifold, go to step
6
If the flow check fails a third time after replacing the manifold, you might have a clog
in the hardware.
a
Inspect the volumes of HT1 in row 1 to make sure that the tubes contain equal
volumes. Higher volumes in the tubes that correspond to lanes with repeated flow
check failure indicate a hardware clog.
b
Unload the run components and perform a maintenance wash.
c
After the wash, turn off the instrument using the power switch. After a few
seconds, turn on the power switch and then press the start button to restart the
software. Power cycling the instrument resets the allowable number of pre-run
check attempts.
d
Follow the software prompts to reload run components and set up your run.
7
If the flow check fails a third time, you can safely bypass the flow check:
a
Select
Bypass Flow Check
to proceed with the run.
b
After the run, check reagent delivery from all tubes.
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