illumina cBot, Системное руководство, Страница: 57 / 90

L
o
a
d
C
o
n
s
u
m
a
b
le
s
cBot 2 System Guide
51
}
All flow cells except HiSeq X and HiSeq 3000/4000 —Remove the flow cell from the
storage tube using plastic forceps. Rinse the flow cell with deionized water and
gently dry with a lens cleaning tissue. Save the tube and buffer for later storage.
}
HiSeq X and HiSeq 3000/4000 flow cells —The patterned flow cell is ready to use
after flow cell preparation.
4 Select Scan Flow Cell ID to activate the external barcode scanner.
5 To scan the flow cell ID, hold the labeled flow cell package or tube close to the scanner
tray with the barcode positioned toward the instrument.
6 Position the flow cell on the thermal stage with flow cell port holes facing upward .
}
HiSeq and HiSeq X flow cells —Lane 1 is on the right side with the keyed corner.
} GAIIx flow cells —Lane 8 is on the right side with the barcode ID.
7 Select the checkbox to indicate that the flow cell is loaded, and then select Next .
Load the Manifold
Use the manifold from the same cluster kit as the flow cell.
1 Inspect the sippers on the sipper comb for damage. Make sure that the black rubber
gaskets are evenly seated.
2 Position the manifold over the flow cell with the sipper comb pointing toward the front
of the cBot.
3 Align the manifold with the guide pins on the thermal stage and seat the manifold
over the flow cell. Seat evenly to form a tight seal.
4 Select the Manifold seated over flow cell checkbox.
5 Close the flow cell clamp to secure the manifold.
Figure 22 Close the Flow Cell Clamp
6 Select the Flow cell clamp closed checkbox.
7 Connect the outlet end of the manifold to the outlet port in the wash reservoir. Make
sure that the outlet is evenly seated.