illumina cBot, Системное руководство

Страница: 39 / 90

L
o
a
d
C
o
n
s
u
m
a
b
le
s
cBot 2 System Guide
33
Load Consumables
From the Load Consumables screen, use the following instructions to load the cBot reagent
plate, flow cell, and barcode labeled 8-tube strip containing prepared libraries. Depending
on the selected protocol, the software prompts you to load an 8-tube strip of additional
primers.
Load the Reagent Plate
1 Remove the clear plastic lid from the cBot reagent plate.
2 Raise the instrument lid by lifting from the cutout on the front of the lid.
3 [For TruSeq v3 reagents] Remove the red foil seal:
a Hold each end of the tube strip in row 10 and peel the red foil from the 8-tube strip.
Discard the foil appropriately.
b Select the onscreen checkbox to indicate that the seal is removed.
4 Pull the reagent plate lever toward you and place the reagent plate onto the reagent
stage with the barcode label facing the back of the instrument.
Figure 12 Position the Reagent Plate
5 Release the lever to secure the reagent plate.
Load the Flow Cell
1 Lift the flow cell clamp.
2 Wash the adapter plate on the thermal stage with a small amount of deionized water,
and then dry with a lint-free tissue. Do not allow fluids to drip inside the instrument.
3 Remove the flow cell from storage:
}
All flow cells except HiSeq X and HiSeq 3000/4000 —Remove the flow cell from the
storage tube using plastic forceps. Rinse with deionized water and then dry with a
lens cleaning tissue using a sweeping motion. Save the tube and buffer for later
storage.
} HiSeq X and HiSeq 3000/4000 flow cells —The patterned flow cell is ready to use
after flow cell preparation.
4 Position the flow cell on the thermal stage with flow cell port holes facing upward .
Lane 1 is on the right side with the keyed corner.