illumina cBot, Системное руководство, Страница: 83 / 90

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cBot 2 System Guide
77
Edit Protocols
Use the Protocol Editor to edit protocols to fit your needs. You might want to repeat steps in
a protocol, or change the number of amplification cycles in the chemistry section.
Each protocol consists of 2 main sections:
} Chemistry section —Contains instructions for pumping reagents, temperature changes,
and wait durations. This section appears in the upper portion of the Protocol Editor
screen.
}
Protocol section —Contains a series of steps made up of chemistry definitions. This
section appears in the lower portion of the Protocol Editor screen.
If you edit an existing protocol, make sure to rename your protocol.
Protocol Editor
1 From the Start screen, select Menu , and then select Protocol Editor .
2 From the Protocol Editor, select Menu , and then select the appropriate command:
} Open —Opens an existing protocol.
}
Select Load from Library —Loads an existing chemistry definition or protocol step
stored in the cBot library.
}
Select New Chemistry Definition or New Protocol Step —Creates a definition or step
and stores it in the cBot library.
3 Use the down arrow to the left of the step to expand the commands in the step. Use the
up arrow to collapse the commands.
4 To edit a step in a chemistry definition, highlight the step.
Selections to change the pump, temperature ramp, or wait commands appear in the
right-hand panel.
5 To edit a step in the protocol, highlight the step.
Selections to change to the number of cycles for the selected chemistry definition appear
in the right-hand panel.
6 Use the Protocol Editor icons to the right of the step name to rearrange, delete, or copy
steps and commands.
Figure 38 Protocol Editor, Expanded Steps