Halma Wagtech Palintest Potatest Go Скачать руководство пользователя страница 16 | Manualshive

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5

Sample Processing - Membrane Filtration

Ensure that at all times you work on the
work-surface included with the kit. Keep this
area as clean as possible and before
starting, wipe it down with a paper towel
and a few drops of methanol.

1

Loosen the filter funnel and remove from
the rubber base support. Invert the filter
funnel and place it down on the clean
work-surface. Also place the blue rubber
base support down on the work-surface.
Ensure that these items are only ever
placed onto the clean work-surface.

2

Sterilise the forceps
by passing them
from side to side
through a flame
from a lighter and
allow to cool. Take
care not to heat
for too long as this
will cause sooty
deposits to form.

3

Remove a sterile, individually wrapped
membrane filter.

4

Peel back the transparent outer wrapper
and use the sterile forceps to separate
the white, gridded membrane filter from
its blue backing paper and remove from
the outer wrapper. Only ever grip the
membrane filters at their edge.

5

Place the membrane filter directly onto the
bronze filter support disc housed in the blue
rubber base; ensure the gridded side is
face-up. If the membrane tears or becomes
contaminated, discard and use a fresh one.

• Membrane Filtration Unit
• Pistol Grip Hand Vacuum Pump
• Forceps/Tweezers
• 47mm Membrane Filters (0.45µm pore size)

• MMD containing Liquid MLSB Media
• Petri Dishes containing Absorbent Pad
• Methanol (not supplied)
• Lighter/Matches (not supplied)

5.0 Membrane Filtration of the Sample

Items Required:

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Содержание Wagtech Palintest Potatest Go

Страница 1: ...Potatest Go Rapid Response Portable Water Quality Laboratory ZI PTW 10005 ...

Страница 2: ...eillance to rapid response testing in an emergency the Wagtech kits provide a robust solution to testing key water quality parameters in the field Acquired by Palintest in 2011 the manufacture and support of the Wagtech portable water quality laboratory range has now been integrated into the Palintest product family Further information regarding the Wagtech product range can be found at www palint...

Страница 3: ...rane Filtration 16 Incubation and Incubator Operation 20 Microbiological Results 31 Water Safety Kit WSK 32 Appendix 1 Field Testing Checklist Hints 37 Appendix 2 Troubleshooting 38 Appendix 3 Technical Specifications 39 Appendix 4 Reagents and Consumables 40 Chapter Page 13 Appendix 5 Microbiological Quick Start Guide 42 ...

Страница 4: ...ater test kit with water safety kit removed from lid and opened to show contents Coloured circles indicate the chapter colour in which their use is explained 1 Kit Layout 28 26 27 29 23 33 24 25 22 12 4 20 2 10 32 8 15 17 16 2 14 19 31 3 1 9 30 34 7 11 13 18 6 5 21 3 4 ...

Страница 5: ...tor 12 Mains Power Supply 13 Battery 14 Battery Leads 15 Car Charging Lead 16 DC Socket 17 DC Cable 18 Incubator Power Cable 19 Microbiological Results Hand Lens 20 Physico Chemical Testing Water Safety Kit WSK 21 Conductivity Solution Mid Range 60mL 22 PH7 Buffer Solution 60mL 23 Chlorine Testing Contour Comparator and accessories 24 DPD 1 3 Reagents 25 Pocket Conductivity Meter 27 pH Testing Poc...

Страница 6: ...t to isolate specific pathogens because they are present in relatively small numbers compared with other types of microorganisms Moreover there are many types of pathogen and each requires a unique microbiological isolation technique The accepted approach is to analyse for indicator organisms that inhabit the gut in large numbers and are excreted in human animal faeces The presence of these indica...

Страница 7: ...ntaminated with harmful pathogens This is especially relevant for the filter membranes absorbent pads and petri dishes that are used in the test For these reasons general hygiene and aseptic procedures are of paramount importance and extra care must be taken when working in the field 2 1 2 Overview of the Procedure for the Microbiological Analysis of Drinking Water The Wagtech kits use classical l...

Страница 8: ...temperatures During this period the Coliform bacteria will multiply rapidly to form colonies that are visible to the naked eye Coliforms are identified by their ability to cause a colour change in the growth media when incubated They will show up as yellow in colour against the red pink background of the media The yellow colonies are counted and the results expressed as Colony Forming Units per 10...

Страница 9: ...ection 3 5 Membrane Filters Tweezers The membrane filters which capture the bacteria during the filtration process are supplied pre sterilised They must be handled using sterile tweezers and never with your fingers 3 0 Preparation Every effort should be made to keep the kit and all its components clean and free from contamination At all times you should work in a manner that limits the chance of c...

Страница 10: ...l burn with a pale blue flame in bright sunlight it can be difficult to see this flame However the heat produced should confirm it has ignited 4 Place the sampling cup base down on a flat surface while the methanol burns 5 Assemble the filter funnel and silicone rubber base components of the MFU Ensure that the filter funnel is inserted in the rubber base in the correct position for sterilisation ...

Страница 11: ...with a clean cloth and place on work surface to dry 2 Attach the absorbent pad cartridge to the dispenser locking it into place 3 Remove the lid of the petri dish and dispense a pad into the dish by sliding back the grooved lever using your thumb 4 Once the pad is safely in the petri dish immediately replace the petri dish lid Take care when handling the petri dish Never touch the internal surface...

Страница 12: ...um hydroxide solution increases pH or dilute hydrochloric acid reduces pH 4 Boil the distilled clean water for 10 minutes and then allow it to cool 5 Ensure that the MMD are sterile before beginning MMDs can be sterilised upright using an autoclave Screw on the lid so that it is secure but not completely air tight to prevent high pressure developing Take care not to place the MMD directly onto the...

Страница 13: ... boiling water for 15 minutes for a third and final time The MLSB media should now be sterile 3 8 Storage of Liquid Media The sterile MLSB should be stable for up to 6 months if stored in a refrigerator If no refrigerator is available the media can be stored for up to 3 months if kept in a dark place away from extremes of heat and moisture However if there are any signs of contamination e g yellow...

Страница 14: ...he growth media MLSB is a differential media which means it can distinguish one microorganism type from another growing on the same media It uses the biochemical characteristics of a microorganism growing in the presence of specific nutrients or indicators such as Phenol Red added to the medium to visibly indicate the defining characteristics of a specific microorganism MLSB is the growth medium f...

Страница 15: ...etween sample collection and analysis is between 2 and 6 hours chill the sample rapidly to about 4 C with ice blocks in an insulated container cool bag Resuscitate the sample prior to full incubation using the automated routine contained within the Wagtech incubator software Even if the sample is kept cold the maximum sample storage time is 6 hours Analysis of samples not stored under these condit...

Страница 16: ...t for too long as this will cause sooty deposits to form 3 Remove a sterile individually wrapped membrane filter 4 Peel back the transparent outer wrapper and use the sterile forceps to separate the white gridded membrane filter from its blue backing paper and remove from the outer wrapper Only ever grip the membrane filters at their edge 5 Place the membrane filter directly onto the bronze filter...

Страница 17: ...are is needed to prevent sample spillage from the filter funnel when assembling NOTE If the sample is not to be collected in the sampling cup part of the membrane filtration unit then the filter funnel base assembly can be inserted into position in the sampling cup straight away 10 Connect the hand vacuum pump and silicone tubing to the MFU 11 Use the pump to create a vacuum and commence the filtr...

Страница 18: ...he work surface 15 Take an MMD containing liquid MLSB media Shake well then remove the blue screw lid and place it lid down onto the work surface 16 Lift the petri dish containing the absorbent pad and hold between thumb and forefinger 17 Take the MMD containing the liquid MLSB and carefully pour the media onto the absorbent pad in the petri dish in a single decisive pouring motion Always ensure t...

Страница 19: ...tiple sample sites Try to conduct all sample processing within a three hour window This ensures a maximum resuscitation period of four hours The resuscitation period is especially relevant for water samples where the environmental exposure is due to chlorination The incubator operating software includes the capacity to have an initial resuscitation period as part of the standard incubation cycle 5...

Страница 20: ... terminals on the battery and plug the cable into the right hand side of the incubator lid This is advisable in areas where the mains supply may not be reliable In the event mains power fails the battery automatically provides power thus continuing the incubation cycle Turn on the incubator by pressing the POWER button briefly 6 1 2 Powering the Incubator via the 12V DC Rechargeable Battery Only T...

Страница 21: ...cycle then follow these steps 1 Ensure the incubator is connected to the 12V battery inside the case 2 Ensure that a reliable 12V power supply is available from either the Power Supply Unit Car Charger Cable or independent Battery Lead 3 Remove DC cable from the left hand side of the incubator 4 Plug in either the Power Supply Unit Car Charger cable or independent Battery Lead to provide 12V DC po...

Страница 22: ... rear of the case 4 Insert the incubator back into the case 5 Plug in the battery cable into the right hand side of the incubator 6 Unplug the Power Supply Unit Car Charger Cable or independent Battery Lead 7 Plug the DC cable into the left hand side of the incubator 8 Plug in either the Power Supply Unit Car Charger Cable or independent Battery Lead into the DC socket on the rear of the case 9 Cl...

Страница 23: ...ll illuminate automatically on key press The initial screen is the Mode page and offers four options Navigation between the options is carried out using the UP DOWN buttons The four options are Incubation select this option to carry out an incubation Voice Instructions select this option to hear useful audio instructions for key steps Used with the prompt cards included Setup view or set User ID T...

Страница 24: ...ssing DONE briefly To modify an existing User ID highlight the ID and press OK briefly Choose Edit and use the UP DOWN keys to select the required alphanumeric characters To remove a character press Back or briefly press Back to allow editing of the right hand character Once complete press DONE briefly to return to the Setup menu To delete an existing User ID highlight the ID and press OK briefly ...

Страница 25: ...o performance monitoring It is possible but unlikely that either thermistor may be damaged in normal use but the possibility of both thermistors being compromised in an identical manner is almost zero The Check Calibration function provides a simple electronic check that both thermistors are reading the same temperature as each other within a specified tolerance If the comparison is out of range t...

Страница 26: ... Menu The Wagtech Incubators all feature a high volume audio speaker to deliver audio prompts These prompts are used in conjunction with the included Prompt Cards Together the two provide clear and concise instruction to key stages of the test procedure There are five standard prompt cards with corresponding audio both indexed by letter and number The Prompt Cards are grouped as follows A0 A6 Prep...

Страница 27: ...art the incubation The screen will scroll between two displays The first displays time remaining in hours and current temperature The second screen displays Sample User ID and incubator cycle status The incubator has a default warm up period of 30 minutes at the start of the incubation process During this phase the incubator will display warming up Once the incubation cycle is complete the incubat...

Страница 28: ... in reports Before selecting function click appropriate flag to change language if required 6 1 Firmware Update The currently installed incubator software version is shown on start up and can also be found in Setup Version see section 6 3 2 If the installed software requires update this can either be carried out via an approved service partner or by downloading the latest firmware from www palinte...

Страница 29: ...instructions included in the incubator support new and experienced users alike in providing simple support for key steps in microbiological analysis Audible prompts are included in English French Spanish and Chinese Mandarin New prompts in local languages and dialects can be uploaded using the Pota Pal app and Audacity software functionality The Android app also has the ability to record prompts T...

Страница 30: ...gnificant period Remove from direct sunlight or high temperature conditions and accept the error Error 107 Validation The internal validation of thermistor temperature monitoring has exceeded the tolerance of the device Return to authorised service centre for attention as soon as possible Error 110 Battery Low The 12V DC battery has insufficient battery capacity to complete the full incubation cyc...

Страница 31: ... but as clumps or in association with particulate matter When enumerating bacteria in water it is not the number of individual bacteria present which are counted but the number of clumps of bacteria or the particles and their associated bacteria Each clump or particle may have many bacteria associated with it Membrane filtration and colony count techniques assume that each bacterium clump of bacte...

Страница 32: ...quick and simple to carry out and the results can give a strong indication of the likely microbiological quality of the water source Because of this it makes sense to conduct these tests first of all before a full microbiological analysis is carried out which is more complex and time consuming The WSK has been designed to complement the increased emphasis now being placed upon Water Safety Plans W...

Страница 33: ...ements Remove the protective cap and press ON OFF to switch the meter on Dip the electrode into 2 3cm of sample rotate gently and wait for the reading to stabilise The protective cap is a useful sample container Note the reading or press HOLD ENT to store the reading on the display Press HOLD ENT to return the display to normal mode After measurement rinse the electrode with clean water Store the ...

Страница 34: ... stains on the electrodes Rinse thoroughly with de ionised water and shake to dry Press ON OFF key to switch on the tester 8 3 2 Range Selection 1 Switch off the tester Press and hold C F key and then switch on the tester using ON OFF key Release C F key 2 The LCD shows the currently selected Range the default is AUTO in the lower display The upper display shows the maximum possible reading for th...

Страница 35: ... the acceptable calibration standard range Use INC and DEC keys to adjust the upper display to the correct conductivity value of the calibration solution 4 Wait for 5 seconds for the tester to automatically confirm the calibration by displaying CO and return to the measurement mode 8 3 6 Temperature Calibration Temperature calibration need not be performed every time unless the temperature reading...

Страница 36: ...tube Add one DPD 1 to the sample tube and crush to a paste Add sample to the tube to the 10ml mark and dissolve any remaining particles Place the tube in the measurement position Fill the second tube with sample and place in the blank position Facing a good light source preferably northerly daylight rotate the disk until the colours match and record the result from the lower right hand window imme...

Страница 37: ...tion periods Ensure you have considered the following additional items that may be required in addition to the PotatestTM contents Sterilised sampling bottles Wagsac sampling bags Result form log book Methanol Non electric autoclave pressure cooker Cigarette lighter Paper towel Liquid detergent De ionised water Waste disposal bags Cool box icepacks Before leaving for the field ensure the following...

Страница 38: ...lysis Select Back to change power source prior to starting incubation Error 111 Battery Critical Battery has 10 minutes life remaining Find a replacement power source immediately Select Back to stop incubation until more reliable supply is available The message will remind at 1 minute intervals until a new power supply is attached Error 112 Power Loss The message will be shown on start up of the i...

Страница 39: ...nd only use genuine Palintest cables chargers Using the PotatestTM incubator in a manner not specified by Palintest may affect safe performance and invalidate warranty Test Protocols 37 and 44 C temperature selections user selectable time period adjustable automatic resuscitation period option Temperature Stability 0 1 C Temperature Control Laser trimmed thermistor pair with automatic temperature ...

Страница 40: ...W 10402 Bronze Disk 7 PTW 10403 Silicone Tubing for MFU 6mm OD 7 PTW 10404 Sample Cup 7 PTW 10405 MFU Silicone Tubing Connector 7 PTW 10412 Forceps 10 PTW 10416 Hand Lens 20 PTW 10700 Pasteur Pipettes 1ml pack of 5 9 PTW 19884 Pen 32 Membrane Filtration Spares Membrane Filtration Consumables Part Number Description PTW 10060 NutriDisk pack for Faecal Streptococci 100 pack PTW 10062 NutriDisk pack ...

Страница 41: ...TW 10438 2 Part Turbidity Tube 28 PT 521 5 Comparator Cuvettes pack of 5 24 PT 663 Cuvette Brush 24 CKD 1001 Contour Comparator Disc Chlorine 0 5mg litre Cl2 24 CKD 1001 Contour Comparator Disc Chlorine 0 1mg litre Cl2 24 PT 502 Crush stir Rods pack of 10 24 66109032 Dilution Tube Sample Tube 29 CKB 1001 Contour Comparator Body 24 143 284 Water Safety Kit Bag 21 12 Appendix 4 Reagents and Consumab...

Страница 42: ...n a bright pink colour Close the lid and then shake to dissolve all the powder Add 10 spoonfuls of culture media into measuring tube using the integral spatula Sterilise the Media Measuring Device at 121 C for at least 10 minutes Fill the Media Measuring Device with the cooled sterilised water to the bottom lip 42 ...

Страница 43: ...flame is nearly extinguished invert funnel and rubber insert into the cup Hold the sampling cup to face away from you Use a lighter to ignite the methanol Rinse the sterile sampling cup with sample water before use Push the filter funnel into the blue rubber base leaving sterilisation symbol visible Wait 15 minutes 15min 43 ...

Страница 44: ... by sliding the grooved lever of the dispenser Replace petri dish lid to keep contents sterile and free from contamination Remove lid of sterile petri dish taking care only to handle the lid by the sides Ensure the absorbent pad is saturated with a small excess of media at the edges Remove the lid from the tube of absorbent pads and attach to the pad dispenser C1 C2 C3 C4 C5 C6 44 ...

Страница 45: ... petri dish Sterilise forceps tips by passing them through flame from a lighter Allow them to cool Place the membrane filter directly onto the bronze filter support disc ensuring the gridded side is face up Lock membrane filter in place by pushing filter funnel firmly down into position in blue rubber base Ensure filter funnel is in the Filtration position Pour water sample into filter funnel up t...

Страница 46: ...ing units per unit volume CFU 100ml Place petri dishes on a flat surface in good natural light Avoid direct sunlight Use the grid on the paper to help count colonies systematically After incubation remove petri dishes Note incubation temperature and period Remove lids Count yellow colonies more than 1mm diameter Use lens if necessary 46 ...

Страница 47: ...acific T 61 1300 13 15 16 E palintest palintest com au A 1 53 Lorraine Street Peakhurst Business Centre Peakhurst NSW 2210 Australia P R Palintest China T 86 10 6588 6200 E china palintest com A Room 1711 Fanli Mansion 22 Chaowai Street Chaoyang District Beijing 100020 PRC Palintest USA T 1 720 221 6878 E info palintestusa com A 400 Corporate Circle Suite J Golden CO 80401 USA 47 ...

Страница 48: ...www palintest com ...

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