Halma Wagtech Palintest Potatest Go, Руководство пользователя

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9
3.1 Aseptic Procedure
There are specific techniques and methods
that actively assist the user in trying to keep
things clean and sterile. These are known
as aseptic procedures or techniques.
This applies most importantly to the following
items in the Wagtech test kit:
Membrane Filtration Unit:
The internal surface of the sample cup, the
internal surface of the funnel and the filter
base/bronze disc must be sterile before a
microbiological test is carried out (section 3.2).
Petri Dishes:
The internal surfaces of the petri dishes will
come into direct contact with the growth
media during the microbiological test. They
must be free from bacteria when the test
begins. They can be sterilised in a variety
of different ways (section 3.3).
Absorbent Pads:
The absorbent growth pads provide a
platform for the liquid growth media and
filter membrane which sit in the petri
dish. There are specific instructions as to
how they must be handled during the
microbiological test (section 3.4).
Culture/Growth Media:
When preparing the media used for the test
you must ensure that the water used to
hydrate the powdered media is sterile. All the
vessels used to prepare the media and into
which the media is dispensed must also be
sterilised in a specific manner (section 3.5).
Membrane Filters/Tweezers:
The membrane filters which capture the bacteria
during the filtration process are supplied
pre-sterilised. They must be handled using
sterile tweezers and never with your fingers.
3.0 Preparation
Every effort should be made to keep the kit and all its components clean and free from
contamination. At all times you should work in a manner that limits the chance of cross
contamination of your samples.
3
Preparation
Useful to Know
• Methanol is highly flammable and is
classed as ‘Dangerous’ for shipping
purposes. For this reason it is not
included as standard in the kit
• Methanol can be supplied separately from
the kits but the freight and associated
hazard charges may be expensive
• As an alcohol it can be challenging
to find methanol in certain countries.
Pharmacies, laboratories and hospitals
are all possible local sources
• When methanol burns in the low oxygen
conditions present in the sample cup of
the MFU, a gas called formaldehyde is
produced. This gas acts as a powerful
disinfectant and ensures a complete
sterilisation of the entire apparatus
• ONLY Methanol can be used to sterilise the
MFU in the field. Ethanol or Methylated
Spirits are not acceptable as they do not
produce formaldehyde when ignited
• To ensure that the MFU is always ready
for use it is a good idea to sterilise it
after each analysis has been performed.
The MFU must however be kept in sterile
condition until the next time it is used