Hach Eclox, Руководство пользователя

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16. Put the pipette tip into the cuvette and remove the pipette from the cuvette to
remove any drops from the outside of the tip.
17. Remove the lids from the CT Reagents 1, 2 and 3.
18. Put a yellow pipette tip on to the yellow pipette.
19. Put 100 µL of CT Reagent 1, 2 and 3 into the cuvette using the pipette. Use a
new pipette tip for each reagent.
20. Open the lid of the luminometer.
21. Lift the cuvette from the holder and gently tap the cuvette two times to mix the
solution.
22. Immediately put the cuvette into the luminometer cell and close the lid.
23. Push PROCEED .
The luminometer automatically starts measuring. After four minutes, the screen
count down timer displays DONE.
24. When the measurement is complete, remove the cuvette from the luminometer.
25. Put the solution in the cuvette into the waste bottle.
26. Put the cuvette into the waste bag.
27. If the reference is between 300 and 900, the calibration is complete.
28. If the reference is not between 300 and 900, push PROCEED and do the
calibration procedure again.
Note: New reagents may give a signal over 900. If the signal is 900 or over, change the
measurement range to the 0–2000 range. Do not throw away the reagent set. If the signal is
below 300, the reagents are probably unusable due to temperature sensitivity and new ones
are required.
Note: If the reagent baseline is reading over 1000 or the luminometer shows Detector
Overload, change the measurement range to the 0–2000 range and continue. There is no
need to throw away the chemiluminescent reagents.
29. If the signal is below 300 again, add another 100 µL of CT Reagent 3 to the
cuvette and do the calibration procedure again.