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Section 4 Chemiluminescence Toxicity
Test
The Chemiluminescence Toxicity Test uses the luminometer. Before doing the
Chemiluminescence Toxicity Test, read section 3.1, Overview on page 15 and do
the procedures in section 3.2, Prepare the luminometer for use on page 16 .
4.1 Overview
The Chemiluminescence Toxicity Test and Luminescent Bacteria Toxicity Test both
show the inhibitory effects of a sample. However, the Chemiluminescence Toxicity
Test reagents are more rugged than the Luminescent Bacteria Toxicity Tests
reagent and can be used under conditions where the Luminescent Bacteria Toxicity
Tests reagent cannot be used.
The Chemiluminescence Toxicity Test reagents are stable for months even if stored
under higher ambient temperatures up to 40 °C ( Table 1 ). The Luminescent
Bacteria Toxicity Tests reagent cannot be stored under those conditions.
4.2 Prepare the reagents for luminometer calibration and
sample testing
Prepare the chemiluminescence test (CT) Reagents 2 and 3 at the beginning of
deployment.
The chemiluminescence test (CT) Reagents 2 and 3 are temperature sensitive and
degrade at high temperatures. For long-term storage, store the reagents in their
stable forms. On the first day of testing, prepare the reagents for routine use.
Diluted reagents are stable for 72 hours. The life of the reagents is longer if the
reagents are kept cool (e.g., in a refrigerator) and in a dark place. Before use, let
the reagents get to ambient temperature.
Table 1 Chemiluminescence test reagent stability
Reagent
Refrigerated in
a dark place
Raised Temperatures
(+40 °C)
Reagent 1 12 to 18 months 1 year
Reagent 2 (stable form) 12 to 18 months 6 months
Reagent 2 (diluted form) 12 to 18 months 72 hours
Reagent 3 (stable form) 12 to 18 months 4 months
Reagent 3 (diluted form) 12 to 18 months 72 hours