BIO RAD VERSADOC, Руководство пользователя

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User’s Manual 4-
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4.3.2 Selecting the Application
The Quantity One program uses an application-oriented format to simplify user selection of
collection parameters. To correctly set the parameters for collection, simply click the
SELECT button in Step I (Figure 4.1) and identify the application name matching your
sample type. For detailed acquisition instructions please refer to the Quantity One software
instruction manual.
4.3.3 Placing Samples in the Imaging Chamber
The VersaDoc can accommodate a variety of sample types and sizes. The sample stage is
25 X 25 cm. This configuration supports the acquisition of smaller areas of interest within
very large samples. The imaging area is liquid sealed so wet samples may be placed
directly into the imaging chamber on the UV transilluminator platen. With wet samples you
will find it convenient to use the sample holders provided with your VersaDoc system.
These sample holders have suction cups that hold the sample in place and keep it from
sliding around on the polished glass surface.
Caution: The sample stage area is resistant to most research chemicals but may be
damaged by extended contact with strong acid solutions and organic solvents. When
imaging samples exposed to these chemicals, users should wash the sample stage with
water and wipe dry immediately after imaging.
Samples should be placed in the VersaDoc instrument following the steps outlined below:
1. Open the main door.
2. Visually check that the sample platen is clean. If not, clean using an optical cleaning
solution and a soft lint-free towel or lens cleaning tissue.
3. From the VersaDoc acquisition window select the POSITION function in Step II (Figure
4.1). The image display window will now present a real time image of the sample in the
chamber that refreshes rapidly.
4. Position your sample on the imaging platen, using the software generated alignment grid, to
ensure that it is correctly placed and in the center of the viewing area.
Note: When imaging fluorescent gel samples it is recommended that the sample be removed from the glass
or plastic plates of the gel sandwich for good image quality. The glass and plastic will fluoresce when
exposed to UV light and will contribute to background signal.
Sample/Chemi Tray
It is recommended that the Sample/Chemi tray (Figure 4.2) be used for all small
chemiluminescence samples (8 x 8 cm or less). This tray slides onto the guides on the
each epi-illumination assembly and allows the sample to be placed closer to the camera.
Large chemi samples should be imaged on the platen. To insert and remove the chemi
tray, the door to the enclosure must be fully opened.
Note: When using the VersaDoc for non-chemiluminescence applications the sample tray should be
removed from the enclosure, as it will block sample signal from reaching the CCD camera.
When using white light epi illumination the sample tray should be placed on the dark reflective platen of
the transilluminator to avoid unwanted reflections on your image.