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User's Manual 

•

 4-

2 

 
 

4.3 Detailed Operating Procedures 

 

4.3.1 Opening the Acquisition Window  

 
After starting your computer, open the Quantity One acquisition and analysis program by 
double clicking on the Quantity One icon. 
 
From the FILE menu select VERSADOC to open the instruments acquisition window.  The 
following window will open on the screen. 
 

Note: If the computer cannot establish communications with the VersaDoc a message will indicate this and 
give the user the option of entering a simulation mode. 

 
 

 

 

 
 
 

Figure. 4.1. VersaDoc acquisition screen in Quantity One

. 

Содержание VERSADOC

Страница 1: ...VersaDoc Imaging System User s Manual for Catalog Numbers 1708010 1708011 1708030 1708031 1708140 1708141 1708050 1708051 Copyright 2001 Bio Rad Laboratories Inc Manual Part Number 4000183 Rev E ...

Страница 2: ......

Страница 3: ...and the coverage provided and are aware of your rights and responsibilities One of the responsibilities of system ownership is regular maintenance Following the maintenance instructions provided with this manual will help to keep your system and peripherals functioning optimally and will protect your investment Please also keep in mind that Bio Rad offers a range of comprehensive service agreement...

Страница 4: ......

Страница 5: ...tup 3 2 3 2 1 Unpacking 3 3 3 2 2 Shipping Check 3 3 3 2 3 Coupling the Camera Module to the Enclosure 3 5 3 2 4 Installing VersaDoc 1000 3000 4000 3 6 3 2 5 Installing VersaDoc 5000 3 8 3 2 6 Installing the Epi Illumination Modules 3 12 3 2 7 Electrical and Communication Connections 3 13 3 2 8 Installing Filters 3 21 3 2 9 Installing the Lens 3 23 Section 4 System Operation 4 1 Starting the Versa...

Страница 6: ...ers 5 2 5 2 Replacing lamps in Illumination Sources 5 2 5 2 1 Replacing lamps in the Transilluminator Module 5 2 5 2 2 Replacing Lamps in the Epi illumination Module 5 4 5 3 Lens and Filter Storage 5 5 Section 6 Troubleshooting Technical Information 6 1 Problem Solving Guide 6 1 6 2 Technical Service 6 3 6 3 VersaDoc System Specifications 6 4 6 4 Warranty Information 6 6 6 5 Glossary of Terms 6 7 ...

Страница 7: ...se to a certain item it will be referred to as SELECT xxxx Notes Cautions and Warnings Notes cautions and warnings are used to highlight certain operating procedures and recommendations A note indicates a special procedure an exception to normal operation or something else of specific interest to the reader Notes are preceded by the word Note in italics A caution precedes an operational step that ...

Страница 8: ...a service appointment The instrument should not be modified or altered in any way Alteration of this instrument voids the manufacturer s warranty and may create a potential safety hazard for the user Caution Bio Rad is not responsible for any injury or damage caused by the use of this instrument for purposes other than that for which it is intended or by the modification of this instrument when no...

Страница 9: ...ccessories even when connected to the VersaDoc system Figure 1 1 shows the serial number certification label which is found on the rear panel of the VersaDoc system This label provides manufacturing data and safety compliance information about the instrument Figure 1 1 For easy customer access the instrument serial number information located at two places in your system 1 On the rear of the instru...

Страница 10: ...cedures other than those specified herein may result in exposure to hazardous UV radiation How to Contact Bio Rad Laboratories In the United States you can reach Bio Rad Laboratories at the following numbers For general information Toll free 1 800 4BIORAD 1 800 424 6723 Fax 1 510 741 5802 email lsg techserv us bio rad com For service or technical support Toll free 1 800 424 6723 Fax 1 510 741 5802...

Страница 11: ...ory throughput and eliminate the need for chemiluminescent detection using x ray film Flexible Design The VersaDoc Imaging system has a very flexible design The light tight enclosure can accommodate any of the offered camera modules The user has the option to upgrade to any of the four standard models The standard VersaDoc comes with every feature included The VersaDoc 1000L 3000L 4000L and 5000L ...

Страница 12: ...d for imaging small samples at high resolution The VersaDoc will accommodate most Nikon f mount lenses with a minimum operating distance MOD of 0 65 m It is highly recommended that lenses be purchased through Bio Rad as some Nikon lenses require modification before they can be inserted into the filter wheel housing Use of an unmodified lens may result in damage to either the instrument or lens mou...

Страница 13: ... description of this software package 2 3 Mechanical Description The VersaDoc Imaging System is a modular system and it consists of the following main hardware components See figure 2 2 1 The light tight enclosure which integrates the sliding UV transillumination module two epi illumination modules chemiluminescence tray access to the filter wheel lenses and all the electronics needed for controll...

Страница 14: ...lat Fielding A uniform images are essential for quantitative analysis The VersaDoc Imaging System features a proprietary algorithm that allows elimination of image non uniformities caused by lens illumination source Optimization of the image uniformity is made possible via the Illumination Flat Fielding function in the image acquisition portion of the software NOTE This feature is only for imaging...

Страница 15: ...nch In placing the VersaDoc users should also allow for easy access to the main power switch which is located on the lower right hand side of the system s rear panel The instrument should be placed where there is adequate room to insert the samples into the front of the enclosure and where it can be easily connected to the host computer The maximum distance between the host computer and the instru...

Страница 16: ...ases in the installation of the VersaDoc system 1 The components are delivered to your laboratory 2 The system is installed by a trained Bio Rad representative 3 Users are trained on the operation of the VersaDoc and accompanying peripherals and software by a Bio Rad representative The following steps for setup of the VersaDoc requires approximately two hours Prepare the VersaDoc hardware 1 Unpack...

Страница 17: ...allow clear access to the rear panel for connection of the appropriate cables Warning Get a helper a single person should not attempt to lift the VersaDoc Warning To avoid back injury always bend your knees and keep a straight back when lifting heavy objects Caution Do not supply power to the instrument until the VersaDoc system has been set up using the following installation procedures 5 Open al...

Страница 18: ... model 3000 4000 and 5000 are shown later in this section 1 VersaDoc transilluminator module already installed inside the light tight enclosure 1 Pair of UV epiilluminator modules 1 Pair of White epi illuminator Modules 1 Sample chemi tray 1 Camera power cable VersaDoc 1000 and 4000 only Or Fan assembly power cable VersaDoc 5000 only 1 Camera AIA interface cable 1 Serial USB communication cable ...

Страница 19: ...available for instrument warranty shipping 3 2 3 Coupling the Camera Module to the enclosure This applies to all camera modules VersaDoc 1000 3000 4000 and 5000 Follow the procedure outlined below to mount the camera module to the enclosure 1 Leave the enclosure on the floor for easy access to the camera mount 2 Correctly position the camera module on the camera mount located at the top of the enc...

Страница 20: ...osures that are serial number XXXBR 300 or greater come with a separate power supply for the camera This power supply already includes a power cable for the VersaDoc 3000 camera VersaDoc 3000 systems Connect this cable between the camera power socket on the camera module and the enclosure Picture shown below is for enclosures with serial numbers below XXXBR0300 VersaDoc 1000 and 4000 Camera Power ...

Страница 21: ...he cable from camera power supply Figure 3 2 NOTE The VersaDoc 4000 camera must be connected to the same power port as the VersaDoc 1000 Connecting the AIA CABLE For VersaDoc 1000 and 3000 and 4000 only 1 Connect the AIA cable Part 800 0247 to the AIA port on the camera See Figure 3 4 Push the cable to the connector such that it clicks and snaps in place properly Figure 3 4 AIA CONNECTORS ON EACH ...

Страница 22: ...wn in the section 3 2 3 above See Figure 3 1 for details 3 Next connect the CEU to the camera Caution The default voltage setting on the camera power supply module is 120 VAC You must follow the instructions below to make sure proper voltage selection is made and fuse is used for you AC voltage environment Caution The VersaDoc 5000 camera module has a BLUE plug installed on the connector on the ca...

Страница 23: ... 3 Pull out the small tab near the AC inlet using a small screwdriver See Figure 3 8 4 The TAB holds a fuse completely remove it This will expose a tiny PCB located in a tiny slot as shown in Figure 3 9 White plastic stub shows the current voltage setting Make sure it matches with the local voltage Insert a small screw driver and pull out the tab AIA Connector on CEU Camera Controller Cable Connec...

Страница 24: ...e Fuse holder inlet 7 Next remove the Phillips screw that holds the fuse holder and turn the holder around so that the two 1 amp fuses face towards the socket See Figure 3 11 Fuse holder Small PCB in a tiny slot This side of the PCB has letters on this side 100 VOLT and arrow must point inward Insert the board back into the socket with the letters facing the right side Plastic stub that shows thro...

Страница 25: ...cable between the camera and the CEU See figure 3 12 VesaDoc 5000 Camera Module Blue Shorting plug Figure 3 12 3 Connect the AC power cord to the AC inlet on the CEU but do not power up the CEU yet 4 For units serial number below XXXBR300 Connect the fan power cable from the fan assembly 5000 models only to the DB9 power connector located at the top surface of the electronics module See picture 3 ...

Страница 26: ... cable to the connector labeled UV TRANSILLUMINATOR located on the ballast panel on interior rear wall of the enclosure 3 To install the epi UV and white modules first install the epi UV module by inserting the two prongs on its end plate to the slots located at the back wall 4 Secure the module using the captive screw by lining up the front side of the module with the hole located in the wall of ...

Страница 27: ...nstall the access panel to the ballast panel NOTE The plugs for each illumination module are keyed and cannot be interchanged Each plug will snap together only to the appropriate socket 3 2 7 Electrical and Communication Connections Power The power entry module of the VersaDoc system is configured for operation at 100 120 VAC 60 Hz or 220 260 VAC 50 Hz After checking that the system s power switch...

Страница 28: ... connected to the host computer via a Serial or USB interface and an AIA interface One each of the Serial and USB port is located on the rear right side of the unit Use the supplied USB or Serial Cable to connect the PC to the VersaDoc Caution To prevent damage to the hardware all instruments must be turned off before attempting to connect or disconnect the unit to the host computer Upon insertion...

Страница 29: ...e connected Software Installation Please follow the instructions below to install the PC or Mac software for VersaDoc system NOTE Do not install the PCI card into the PC yet First install the drivers as shown below and then install the PCI Card PC based VersaDoc systems a Insert The Discovery Series software CD into the CD ROM drive of the PC It will go into AUTO RUN mode and the following window ...

Страница 30: ... the arrow next to ChemiDoc XRS VersaDoc drivers to get installer options See picture below d If you are installing the drivers on local drive select appropriate option by scrolling down as shown in the picture below Click on NEXT ...

Страница 31: ...go through a FOUND NEW HARDWARE installation routine and find the drivers that were just installed NOTE In case the PCI card was installed before the software then go to section 6 for software troubleshooting In case of the MAC systems it does not matter if the PCI Card is installed before or after installing the software and drivers Mac based VersaDoc systems NOTE This procedure applies to both M...

Страница 32: ... and click on INSTALL The following window will open j Depending upon the Imaging device you are installing click on the desired Imager you want to install and then click on Install The drivers will get loaded and at the completion of the installation the following window will open ...

Страница 33: ...e that the VersaDoc system is turned off before Installing the digitizing card and AIA cable 1 Now install the digitizing card into a PCI slot in your computer and secure it with appropriate screw See Figure 3 17 PCI digitizing card PCI slot inside the PC Figure 3 17 2 Connect the camera to the digitizing card with the AIA cable Do not power up the PC MAC yet See section 3 2 9 for powering up the ...

Страница 34: ...c 5000 System under Serial xxxBR300 Figure 3 18c VersaDoc 3000 Systems starting Serial xxxBR300 Figure 3 18d VersaDoc 5000 System starting Serial xxxBR300 Figure 3 18e VersaDoc 1000 and 4000 Systems starting Serial xxxBR300 AIA Cable Power Cable Serial USB Control Cable Fan Cable Serial USB Power Supply AIA Cable Camera Power Cable Serial USB AIA Cable Power Cable AIA Cable Serial USB Fan Cable Se...

Страница 35: ... Used for most single color fluorescent stains and labels 2 530BP Band Pass Used for detection of green signal FITC etc in multi color fluorescence experiments 3 610LP Long Pass Used for detection of red signals Texas Red etc in multi color fluorescence experiments 4 Clear Used for system focus and some colorimetric applications To install the filters follow the procedure below Figure 3 19 1 Ensur...

Страница 36: ...lter wheel position 7 Repeat steps 4 5 for the remaining filters 2 3 4 8 Replace the filter wheel cover and hand tighten both captive thumbscrews 9 Close the main door firmly The filter wheel will automatically reset to the home position and is now ready for operation NOTE The above procedure can also be used for the installation of custom filters Open filter wheel access panel and rotate it downw...

Страница 37: ...the sample closer to the camera Infrared Cutoff Filter When performing any fluorescence experiments it is required that the 660 nm infrared cut off filter that is supplied with the VersaDoc is installed on the front of collecting lens This filter will block any infrared signal that will be generated by the UV bulbs substantially reducing image background and improving sensitivity This filter is no...

Страница 38: ...e platen cover can be removed Figure 3 20 Steps in zoom lens installation High Sensitivity 50 mm Lens Installation To correctly install the 50 mm high NA lens follow the procedure outlined below Figure 3 21 1 Remove the lens from its packaging and retain the packaging for future storage 2 Remove the protective cover from the lens mount rear of lens and check that the lens is clean free of dust fin...

Страница 39: ...ion Accidentally dropping the lens could break the UV transilluminator platen or white light conversion screen Replacement of such a broken platen or white light conversion screen is not covered by warranty or service contract 3 Depress the red release button on the base of the camera housing This is located to the rear right hand side of the lens 4 Rotate the lens in a clockwise direction to the ...

Страница 40: ...User s Manual 3 26 Figure 3 22 Steps in lens removal Proceed to section 4 ...

Страница 41: ...VersaDoc acquisition window cannot be opened on the host computer please contact the Bio Rad Technical Service Department for assistance The VersaDoc should be switched on at least 30 seconds before the host computer to allow for complete initialization If the VersaDoc is not fully operational before the computer is turned on the system will not be recognized as an attached Serial USB device and t...

Страница 42: ...double clicking on the Quantity One icon From the FILE menu select VERSADOC to open the instruments acquisition window The following window will open on the screen Note If the computer cannot establish communications with the VersaDoc a message will indicate this and give the user the option of entering a simulation mode Figure 4 1 VersaDoc acquisition screen in Quantity One ...

Страница 43: ...g the steps outlined below 1 Open the main door 2 Visually check that the sample platen is clean If not clean using an optical cleaning solution and a soft lint free towel or lens cleaning tissue 3 From the VersaDoc acquisition window select the POSITION function in Step II Figure 4 1 The image display window will now present a real time image of the sample in the chamber that refreshes rapidly 4 ...

Страница 44: ...ll produce superior images for all low intensity chemiluminescence experiments The lens can also be used for collecting typical fluorescence and colorimetric images however the imaging area is fixed This lens is designed to work in combination with the chemiluminescence sample tray placing the sample closer to the camera for improved light collection efficiency Infrared Cutoff Filter When performi...

Страница 45: ... ring on the 50 mm lens will not lock in the fully closed f stop 16 position unless the user activates the lock mechanism on the lower right hand side of the f stop indicators To lock in this position move the button white dot up so that it aligns with the orange indicator It is not recommended that the lens be locked in this position for chemiluminescence experiments as this setting allows the le...

Страница 46: ...nger the exposure time the brighter the captured image will be For high intensity applications including colorimetric and high intensity fluorescence experiments an exposure time of only a few seconds is typically required For low intensity applications such as chemiluminescence an exposure time of several minutes may be required The exposure period required to produce an optimal image varies cons...

Страница 47: ...rsaDoc Fluorescent Reference plate When your application requires white light transillumination the software will also prompt you to place the VersaDoc white light conversion screen on the UV transilluminator platen This white light conversion screen absorbs the UV from the UV transilluminator and converts this broadband UV into white light See figure 4 5 Figure 4 5 White Light Conversion Screen I...

Страница 48: ... sessions to optimize image quality Use powder free gloves when cleaning the instrument to avoid fingerprints that may appear during imaging Never wear powdered gloves when cleaning the VersaDoc Clean the sample platen with optical cleaning solution and a lint free optical tissue Cleaning kits are available from Bio Rad and one is supplied with the instrument Caution It is recommended that water m...

Страница 49: ...tion time for image acquisition has increased more than three fold it is recommended that the lamps be replaced The life of the white light lamp is approximately 1000 hours NOTE THE LAMPS SHOULD BE REPLACED IN PAIRS FROM ALL ILLUMINATION MODULES 5 2 1 Replacing the Lamps in the Transilluminator Module To access the lamps in the transilluminator follow the procedure outlined below Caution Do not to...

Страница 50: ...iformity of your UV transilluminator 8 Remove lamps by holding the metal part of the lamps with the tip of your fingers and turning them in a rotating movement and by pulling them out of the socket See Fig 5 2 Remove hex standoffs X4 Remove the baffle exposing the lamp Hold at the metal ends of the lamps and turn to remove Figure 5 2 9 Install the replacement lamp by inserting its ends in the sock...

Страница 51: ...cedure outlined below Figure 5 3 NOTE The procedure to remove and replace the lamp in the UV or white light epi modules is the same The procedure outlined below applies to both 1 Turn off the VersaDoc and disconnect all power removing the power cable 2 Open the door to the sample compartment 3 Find the threaded mounting screw that secures the epi modules to the wall of the enclosure and loosen it ...

Страница 52: ...e in its place and reconnect epi cables to the ballast assembly to appropriate connectors 20 Now power up the system and try running the application that use the epi source in which the lamps were installed to make sure that the lamps are operating normally NOTE THE UV INTERLOCK ON THE MAIN DOOR WILL NOT ALLOW UV EPI TO TURN ON ACQUIRE AN IMAGE OF A WHITE PIECE OF PAPER TO ENSURE THAT THE EPI UV L...

Страница 53: ... init file Contact Bio Rad for assistance Image is not visible on the monitor or only low signal counts are detected The Transform function in the software is set too high Set to a lower maximum value Lens cap is covering lens Remove lens cap Insufficient integration time Integrate sample for a longer time Chemi tray not removed when imaging nonchemi sample Remove chemi tray from sample chamber Wr...

Страница 54: ...cable Filter wheel does not turn Poorly seated lamps Turn the lamps in the sockets to make sure lamps are seated properly in their sockets Bad lamps Replace lamps Door interlock not working Contact Bio Rad Epi lamps are not turning on Lamp intensity has decreased with age Replace lamps Incorrect f stop setting Open aperture and reacquire the image Integration time to acquire image has increased In...

Страница 55: ...tility in the CONTROL PANELS 3 Click and highlight the PCI card in question Roper or Scion and click on REMOVE Follow the prompts and reboot the system 4 Windows will now find the card and install the drivers as described above 6 2 Technical Service For technical assistance with the VersaDoc system including all hardware and software contact your local Bio Rad office or in the US call 1 800 424 67...

Страница 56: ...g Dimensions 23 W x 26 D x 39 H Total Weight 96 lbs VersaDoc 1000 Cooled CCD Specification Imaging array 1317 X 1035 Pixel size 6 8 x 6 8 micron Pixel depth 12bit Detector type Scientific grade high sensitivity front illuminated CCD with antireflective coating Cooling system Forced air peltier thermoelectric system Cooling range 10 C 2 C Dynamic Range 3 4 orders Illumination Flat Fielding Yes Vers...

Страница 57: ...rced air peltier thermoelectric system Cooling range 10 C 2 C Dynamic Range 3 4 orders Illumination Flat Fielding Yes VersaDoc 5000 Cooled CCD Specification Imaging array 512 x 512 Pixel size 24 x 24 micron Pixel depth 16bit Detector type Scientific grade high sensitivity back illuminated CCD with antireflective coating Cooling system Forced air peltier thermoelectric system Cooling range 25 to 35...

Страница 58: ...ervice under this warranty shall be requested by contacting your nearest Bio Rad office This warranty does not extend to any instruments or parts thereof that have been subject to misuse neglect or accident or that have been modified or serviced by anyone other than Bio Rad or its representative or that have been used in violation of Bio Rad instructions It also does not extend to instruments or p...

Страница 59: ...gnal to Noise Signal to noise ratio SNR is the measure of the signal quality at a given pixel It is the ratio of the measured signal to the overall measured noise at that pixel Dynamic Range Dynamic range of a CCD is simply defined as the ratio of CCD saturation to the read noise It is the ability to quantitatively detect very dim and very bright pixels within a single image Quantum Quantum effici...

Страница 60: ...cent 1002363 Shipping Container Complete System 1002364 Shipping Container Enclosure Only You will need these in pairs Other Useful Part Numbers Part Number Description 1707813 Sample Holder Kit 1708001 White Light Conversion screen 1708007 Sample Chemi Tray 1708008 Plate Reference Fluorescent 1708012 Camera Module VersaDoc 1000 1708032 Camera Module VersaDoc 3000 1708142 Camera Module VersaDoc 40...

Страница 61: ...x 852 2789 1257 India Ph 91 124 6398112 113 114 Fx 91 124 6398115 Israel Ph 03 951 4124 Fx 03 951 4129 Italy Ph 34 91 590 5200 Fx 34 91 590 5211 Japan Ph 03 5811 6270 Fx 03 5811 6272 Korea Ph 82 2 3473 4460 Fx 82 2 3472 7003 Latin America Ph 305 894 5950 Fx 305 894 5960 Mexico Ph 52 5 534 2552 to 54 Fx 52 5 524 5971 The Netherlands Ph 0318 540666 Fx 0318 542216 New Zealand Ph 64 9 4152280 Fx 64 9 ...

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