BIO RAD VERSADOC, Руководство пользователя

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User’s Manual
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2-4
2.4 Overview of the Imaging Process
The acquisition and analysis of image data using VersaDoc technology is a simple
five-part process.
Step 1: Samples to be imaged are placed on the transilluminator module.
Step 2: The appropriate imaging method is selected in the VersaDoc acquisition
window of Quantity One.
Step 3: The sample is aligned using the positioning template in the Quantity One
acquisition window.
Step 4: If required, the imaging lens is zoomed and focused onto the sample to obtain
the highest quality image.
Step 5: The desired collection time for an aperture setting of the lens is selected and
the image is captured. In cases where Flat Fielding is selected, a reference image is
also acquired (see below). Once the sample image is collected and saved, this
Image can then be reviewed and analyzed using the appropriate tools bundled in the
Bio-Rad software package. Please refer to the software manual for further details.
2.5 Illumination Flat Fielding:
A uniform images are essential for quantitative analysis. The VersaDoc Imaging
System features a proprietary algorithm that allows elimination of image non-
uniformities caused by lens, illumination source. Optimization of the image uniformity
is made possible via the Illumination Flat Fielding function in the image acquisition
portion of the software.
NOTE: This feature is only for imaging applications that utilize a UV or white
light transillumination source.
Illumination Flat Fielding when using the UV transilluminator:
The software takes an initial image and then prompts the user to remove the sample
and to place the VersaDoc Fluorescent Reference Plate provided with your system
on the UV Transilluminator.
Illumination Flat Fielding when using the white light conversion screen:
The software will prompt the user at the beginning of the image acquisition to place
the
VersaDoc white light conversion screen on the UV transilluminator . The sample is
placed on the conversion screen; an initial image is taken. The software then
prompts the user to remove the sample from the VersaDoc white light conversion
screen and then finishes image acquisition
.