BIO RAD VERSADOC, Руководство пользователя

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User's Manual
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3.2.9 Installing the Lens
The VersaDoc system is supplied with two standard lenses, a flexible zoom
lens and a high NA 50-mm lens with improved collection efficiency
Zoom Lens for fluorescence and colorimetric imaging
The Tamron zoom lens is recommended for use with all fluorescence and colorimetric
applications and for high intensity chemiluminescence experiments. The zoom
capabilities of this lens supports the imaging of small and large samples. The zoom lens
is not a high numerical aperture (NA) lens and should not be used for low intensity
chemiluminescence experiments.
High Numerical Aperture Lens for Chemiluminescence Imaging
The Nikon 50 mm high NA (f/1.4) lens is designed for optimized light collection
efficiency and should be used for all low intensity chemiluminescence experiments. The
lens can also be used for collecting typical fluorescence and colorimetric images. The
chemiluminescence sample tray may be used to move the sample closer to the camera.
Infrared Cutoff Filter
When performing any fluorescence experiments it is required that the 660 nm infrared
cut-off filter that is supplied with the VersaDoc is installed on the front of collecting lens.
This filter will block any infrared signal that will be generated by the UV bulbs,
substantially reducing image background and improving sensitivity. This filter is not
required for chemiluminescence experiments and should not be used when collecting
low intensity chemiluminescence signals, as it will reduce the amount of signal
collected.
Lens Use Recommendation
For optimal image acquisition, it is recommended that the zoom lens with 660 nm cut-off
filter installed is used for all fluorescence and colorimetric applications. In
chemiluminescence experiments, the 50 mm fixed lens with no cut-off filter is
recommended.
Zoom Lens Installation
To correctly install the zoom lens. Follow the procedure outlined below (Figure
3.20):
1. Remove the lens from its packaging and retain the packaging for future storage.
2. Remove the front lens cap and install the 58mm-52mm step down ring, then the
660 short pass (SP) filter onto the lens.